Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid
Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid
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DOI：
10.1105/tpc.109.070334
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发表时间：
2009-10-01
期刊：
影响因子：
11.6
通讯作者：
Niks, Rients E.
中科院分区：
文献类型：
--
作者：
Jeuken, Marieke J. W.;Zhang, Ningwen W.;Niks, Rients E.
一些种间和种内杂交可能导致后代的生存力降低或不育，通常是由于两个或多个基因座之间的相互作用导致的遗传不相容性。杂种坏死是一种合子后遗传不亲和性，表现为植物上的坏死病变。我们观察到杂种坏死种间莴苣（莴苣和莴苣）杂交种，与抗霜霉病。分离分析表明，在两个相互作用的基因座是负责一个特定的等位基因组合。等位基因的相互作用有两个结果：（1）定量的温度依赖性自身免疫反应，导致坏死病变，致死性，和定量的阻力，否则毒力种族的莴苣盘梗霉;和（2）定性的温度无关的种族特异性抗性的无毒种族的B。莴苣我们通过瞬时表达和沉默实验证明，两个相互作用的基因之一是Rin 4。在拟南芥中，已知RIN 4与多个R基因产物相互作用，并且它们的相互作用导致对假单胞菌的超敏抗性。对生菜中Rin 4的坏死诱导等位基因进行定点突变研究，结果表明三个残基对杂交坏死至关重要。
Some inter-and intraspecific crosses may result in reduced viability or sterility in the offspring, often due to genetic incompatibilities resulting from interactions between two or more loci. Hybrid necrosis is a postzygotic genetic incompatibility that is phenotypically manifested as necrotic lesions on the plant. We observed hybrid necrosis in interspecific lettuce (Lactuca sativa and Lactuca saligna) hybrids that correlated with resistance to downy mildew. Segregation analysis revealed a specific allelic combination at two interacting loci to be responsible. The allelic interaction had two consequences: (1) a quantitative temperature-dependent autoimmunity reaction leading to necrotic lesions, lethality, and quantitative resistance to an otherwise virulent race of Bremia lactucae; and (2) a qualitative temperature-independent race-specific resistance to an avirulent race of B. lactucae. We demonstrated by transient expression and silencing experiments that one of the two interacting genes was Rin4. In Arabidopsis thaliana, RIN4 is known to interact with multiple R gene products, and their interactions result in hypersensitive resistance to Pseudomonas syringae. Site-directed mutation studies on the necrosis-eliciting allele of Rin4 in lettuce showed that three residues were critical for hybrid necrosis.