Characterization of the circadian oscillator in the choroid plexus of rats
Characterization of the circadian oscillator in the choroid plexus of rats
复制标题
大鼠脉络丛昼夜节律振荡器的表征
DOI：
10.1016/j.bbrc.2020.01.125
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Iijima Norio
中科院分区：
文献类型：
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作者：
Yamaguchi Takeshi;Hamada Toshiyuki;Matsuzaki Toshiyuki;Iijima Norio
昼夜节律是控制各种生理功能的基本生物现象。视交叉上核（SCN）是整合各种外围时钟的主时钟。最近，脉络丛（CP）被报道是这样一个外周时钟，昼夜节律振荡器，可能反过来影响SCN。因此，我们研究的主要目的是解开CP内的昼夜节律振荡器。对strPer 1、rPer 2和rBmal 1的定量PCR显示，侧脑室（CP-LV）和第四脑室（CP-4V）的CP具有强大的昼夜节律振荡器。CP振子的相位介于松果体（PG）和SCN的相位之间。外植体的生物发光监测显示，CP-LV和CP-4V的固有昼夜节律周期约为21 h，比SCN和PG短。CP-LV、CP-4V、PG和SCN的振荡器之间的相互作用可能确保SCN采用稳定的24 h节律，每个区域都有一个具有不同相位和周期的本征振荡器。原位杂交分析表明，rPer 2仅在CP的上皮细胞中表达。此外，CP昼夜振荡器可能控制脑脊液分泌。然而，没有观察到水通道，水通道蛋白1的表达的昼夜变化。需要进一步的研究来阐明昼夜节律对CP的影响。
Circadian rhythms are a fundamental biological phenomena that control various physiological functions. The suprachiasmatic nucleus (SCN) is a master clock that integrates various peripheral clocks. Recently, the choroid plexus (CP) was reported to be one such peripheral clock, a circadian oscillator that might conversely affect the SCN. Hence, the principle aim of our study was to unravel the circadian oscillator within the CP. Quantitative PCR againstrPer1,rPer2, andrBmal1showed that CP in the lateral ventricle (CP-LV) and fourth ventricle (CP–4V) has a robust circadian oscillator. The phases of the CP oscillator are between those of the pineal gland (PG) and SCN. Bioluminescence monitoring of explants showed that the intrinsic circadian period of CP-LV and CP-4V was approximately 21 h, which is shorter than SCN and PG. It is possible that interaction between oscillators of the CP-LV, CP-4V, PG, and SCN ensures the SCN adopts a stable 24 h rhythm, with each of the regions having an intrinsic oscillator with different phases and periods.In situhybridization analysis revealed that dusk-to-dawn variation ofrPer2expression was found in epithelial cells of the CP only. Furthermore, the CP circadian oscillator might control cerebrospinal fluid secretion. However, no dusk-to-dawn variation in expression of the water channel, aquaporin 1, was observed. Further investigations are needed to clarify the involvement of circadian rhythm on CP.