Mechanosensitive Primary Bladder Afferent Activity in Rats With and Without Spinal Cord Transection
Mechanosensitive Primary Bladder Afferent Activity in Rats With and Without Spinal Cord Transection
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DOI：
10.1016/j.juro.2009.07.012
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发表时间：
2009-11-01
影响因子：
6.6
通讯作者：
Wyndaele, Jean-Jacques
中科院分区：
文献类型：
--
作者：
Iijima, Kazuyoshi;Igawa, Yasuhiko;Wyndaele, Jean-Jacques
目的：脊髓损伤引起膀胱传入通路的功能和形态学改变。然而，主要来自膀胱的传入神经活动的兴奋性的变化的直接证据还没有得到明确的证明。因此，我们确定了周围的机械敏感性膀胱传入的盆神经和可能的传入变化A δ和C纤维脊髓injure.Materials和方法：成年雌性大鼠分为2组，包括脊髓损伤和神经系统完整的动物。脊髓损伤组在功能实验前4周于Th9脊髓横断。对于单个单位传入活动监测，从L6背根解剖细丝并鉴定膀胱传入纤维。单单位传入活动进行了研究，在不断填充salines.Results：两个传入模式与小阶段性增加膀胱充盈过程中的膀胱内压，包括加速和nonaccelerated类型。脊髓损伤组中A δ和C纤维的加速型发生率显著高于神经功能完好组。然而，我们没有发现神经系统完整或脊髓injured rats.Conclusions：结果表明，机械敏感的膀胱传入活动有几种模式，并促进脊髓损伤后，特别是在音乐会与小膀胱收缩（微动）的传导速度和膀胱机械敏感传入纤维的功能特性之间的关系。这些单个传入纤维的功能特性与它们的传导速度没有明显的关系，因此可能与传入纤维类型有关。
Purpose: Spinal cord injury induces functional and morphological changes in bladder afferent pathways. However, direct evidence for changes in the excitability of afferent nerve activity primarily originating from the bladder has not been clearly demonstrated. Thus, we determined the characteristics of peripheral mechanosensitive bladder afferents in the pelvic nerve and possible afferent changes in A delta and C fibers after spinal cord injury.Materials and Methods: Adult female rats were divided into 2 groups, including spinal cord injured and neurologically intact animals. In the spinal cord injury group the spinal cord was transected at Th9 at 4 weeks before functional experiments. For single unit afferent activity monitoring fine filaments were dissected from the L6 dorsal root and bladder afferent fibers were identified. Single unit afferent activity was studied during constant filling with saline.Results: Two afferent patterns were linked to small phasic increases in intravesical pressure during bladder filling, including accelerated and nonaccelerated types. The incidence of the accelerated type was significantly higher in the spinal cord injury group than in the neurologically intact group regarding A delta and C fibers. However, we found no relationship between conduction velocity and the functional properties of bladder mechanosensitive afferent fibers in neurologically intact or spinal cord injured rats.Conclusions: Results indicate that mechanosensitive bladder afferent activity has several patterns and is facilitated after spinal cord injury, especially in concert with small bladder contractions (micromotions). The functional properties of these individual afferent fibers are not related in an obvious manner to their conduction velocity and, thus, probably the afferent fiber type.