Factors Affecting Mouse Somatic Cell Nuclear Reprogramming by Rabbit Ooplasms.
Factors Affecting Mouse Somatic Cell Nuclear Reprogramming by Rabbit Ooplasms.
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DOI：
10.1089/cell.2017.0021
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发表时间：
2017-12
影响因子：
1.6
通讯作者：
Xia Huang;Lili Song;Zhiyan Zhan;Haihui Gu;Haizhong Feng;Yanxin Li
中科院分区：
文献类型：
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作者：
Xia Huang;Lili Song;Zhiyan Zhan;Haihui Gu;Haizhong Feng;Yanxin Li
种间体细胞核移植（iSCNT）胚胎的成功发育取决于卵质和核组分之间的相容性。然而，调节相容性的机制仍然未知。本研究以小鼠Oct 4-绿色荧光蛋白（GFP）细胞为供体，兔卵母细胞为受体，在部分小鼠-兔iSCNT胚胎中发现Oct 4等多能性相关基因被重新激活，并激活Oct 4启动子驱动的GFP报告基因表达。串联核移植提高了Oct 4的再激活效率。在GFP阳性的iSCNT囊胚中检测到Oct 4启动子的DNA去甲基化，而GFP阴性的iSCNT胚胎表现出低效率。我们的研究结果表明，Oct 4-GFP可以很好地标记胚胎的表观遗传重构和多能基因表达的再激活。在小鼠-兔重组胚胎的整个植入前阶段，发现了丰富的兔线粒体特异性DNA。我们的数据表明，表观遗传重塑和完整的线粒体匹配对于植入前成功的iSCNT胚胎发育是不必要的。
Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors and rabbit oocytes as recipients, we show that Oct4 and other pluripotency related genes were reactivated in some of mouse-rabbit iSCNT embryos, which could also activate Oct4 promoter-driven GFP reporter gene expression. Series nuclear transfer improved the efficiency of Oct4 reactivation. DNA demethylation of Oct4 promoter was detected in GFP positive iSCNT blastocysts, whereas GFP negative iSCNT embryos showed a low efficiency. Our results demonstrate that Oct4-GFP can well label the embryos with epigenetic remodeling and reactivation of pluripotent gene expression. Abundant rabbit mitochondria specific DNAs were identified in reconstructed mouse-rabbit embryos throughout preimplantation stages. Our data demonstrate that epigenetic remodeling and the complete mitochondrial match are not necessary for successful iSCNT embryo development before implantation.