Up-regulation of cyclooxygenase-2 mRNA in the rat spinal cord following peripheral inflammation
Up-regulation of cyclooxygenase-2 mRNA in the rat spinal cord following peripheral inflammation
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DOI：
10.1016/0014-5793(96)00604-7
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发表时间：
1996-07-22
期刊：
影响因子：
3.5
通讯作者：
GoppeltStruebe, M
中科院分区：
文献类型：
--
作者：
Beiche, F;Scheuerer, S;GoppeltStruebe, M
前列腺素（PG）被描述为外周炎症后脊髓伤害性处理的介质。PG生物合成所必需的酶，环氧合酶同工酶考克斯-1和考克斯-2，尚未在脊髓中进行研究。在两项对具有促炎剂诱导的外周炎症的大鼠的研究中，使用逆转录-聚合酶链反应（RT-PCR）技术分析了脊髓腰段中两种考克斯同工酶的mRNA表达水平。我们可以表明，两种考克斯亚型的mRNA在脊髓中组成性表达，考克斯-2作为主要亚型。诱导外周炎症后6小时，考克斯-2 mRNA表达水平相对于未处理的对照大鼠显著升高，并在诱导炎症后3天内恢复到基线，因此，考克斯-2可能被认为是在外周炎症刺激下负责脊髓PG释放的考克斯同工酶。
Prostaglandins (PG) have been described as mediators in spinal nociceptive processing after peripheral inflammation. Enzymes essential for PG biosynthesis, cyclooxygenase isozymes COX-1 and COX-2, have not yet been investigated in the spinal cord, In two studies on rats with adjuvant-induced peripheral inflammation levels of mRNA expression of both COX isoforms were analyzed in the lumbar section of the spinal cord using reverse transcription-polymerase chain reaction (RT-PCR) technique. We could show that mRNA of both COX isoforms is expressed constitutively in the spinal cord with COX-2 as the predominant isoform, Six hours after induction of peripheral inflammation, levels of COX-2 mRNA expression were raised significantly in respect to untreated control rats and returned to baseline within 3 days after induction of inflammation, COX-2 might therefore be regarded as the COX isozyme responsible for spinal PG release in nociceptive processing under a peripheral inflammatory stimulus.