Chemotaxis of large granular lymphocytes.
Chemotaxis of large granular lymphocytes.
复制标题
大颗粒淋巴细胞的趋化性。
作者：
B. Pohajdak;J. Gómez;F. Orr;N. Khalil;M. Talgoy;A. Greenberg
验证了大颗粒淋巴细胞（LGL）具有定向运动（趋化性）的假设。从不连续的Percoll梯度中分离出来的LGL群体沿着n-甲酰基-蛋氨酸-亮基-苯丙氨酸（f-MLP）、酪蛋白和C5a（众所周知的多形核白细胞和单核细胞的化学引诱剂）、干扰素- β和集落刺激因子的浓度梯度迁移。白细胞介素2、凝血因子、血小板衍生生长因子和纤维连接蛋白均无活性。在具有最高裂解活性的Percoll组分和HNK-1+细胞中，迁移反应更大。当趋化剂浓度较高时，f-MLP、酪蛋白和C5a的趋化反应总是较大的。在使用12微米硝化纤维素过滤器孵育1小时后，观察到最佳的趋化性。当允许LGL迁移较长时间（大于2小时），以及在体外培养24至72小时时，在含有植物血凝素条件的培养基中存在或不存在IL - 2时，LGL表现出高度的非定向运动。根据单克隆抗体和补体耗损，趋化性LGL为HNK-1+、OKT11+或HNK-1+、OKT11-。它们没有T细胞或单核细胞表面标记，表现出OKT3-， OKT4-, OKT8-， OKM1-和MO2-表型，并且在29℃下没有形成E型莲座，这是溶解性NK细胞与T细胞相比的特征。此外，大鼠LGL白血病（RNK）对f-MLP和酪蛋白均表现出趋化反应。LGL对f-MLP的趋化性可被无活性结构类似物cbz -ph -met以剂量依赖性的方式抑制，而RNK肿瘤系特异性结合f-ML[3H]P，表明LGL具有趋化肽受体。
The hypothesis that large granular lymphocytes (LGL) are capable of directed locomotion (chemotaxis) was tested. A population of LGL isolated from discontinuous Percoll gradients migrated along concentration gradients of N-formyl-methionyl-leucyl-phenylalanine (f-MLP), casein, and C5a, well known chemoattractants for polymorphonuclear leukocytes and monocytes, as well as interferon-beta and colony-stimulating factor. Interleukin 2, tuftsin, platelet-derived growth factor, and fibronectin were inactive. Migratory responses were greater in Percoll fractions with the highest lytic activity and HNK-1+ cells. The chemotactic response to f-MLP, casein, and C5a was always greater when the chemoattractant was present in greater concentration in the lower compartment of the Boyden chamber. Optimum chemotaxis was observed after a 1 hr incubation that made use of 12 micron nitrocellulose filters. LGL exhibited a high degree of nondirected locomotion when allowed to migrate for longer periods (greater than 2 hr), and when cultured in vitro for 24 to 72 hr in the presence or absence of IL 2 containing phytohemagluttinin-conditioned medium. The chemotactic LGL was HNK-1+, OKT11+ or HNK-1+, OKT11- on the basis of monoclonal antibody and complement depletion. They did not bear either T cell or monocyte cell surface markers, exhibiting an OKT3-, OKT4-, OKT8-, OKM1-, and MO2- phenotype, and did not form E rosettes at 29 degrees C, which is characteristic of lytic NK cells in contrast to T cells. Furthermore, a rat LGL leukemia (RNK) exhibited a chemotactic response to both f-MLP and casein. LGL chemotaxis to f-MLP could be inhibited in a dose-dependent manner by the inactive structural analog CBZ-phe-met, and the RNK tumor line specifically bound f-ML[3H]P, suggesting that LGL bear receptors for the chemotactic peptide.