In vivo tomographic imaging of red-shifted fluorescent proteins.
In vivo tomographic imaging of red-shifted fluorescent proteins.
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DOI：
10.1364/boe.2.000887
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发表时间：
2011-03-16
影响因子：
3.4
通讯作者：
Ntziachristos V
中科院分区：
文献类型：
--
作者：
Deliolanis NC;Wurdinger T;Pike L;Tannous BA;Breakefield XO;Weissleder R;Ntziachristos V
我们开发了一种用于动物远红和近红外荧光蛋白三维断层扫描的光谱反演方法。该方法是专门为解决血红蛋白从可见光到远红光发生在600纳米左右的陡峭光吸收转变而开发的。利用表达红移荧光蛋白mCherry的原位脑肿瘤小鼠模型，我们证明了与单波长全身重建相比，成像精度有显著提高。此外，我们显示在灵敏度的改善至少一个数量级的绿色荧光蛋白（GFP）的全身成像。我们讨论了使用进一步的红移荧光蛋白如何获得额外的灵敏度增益，并解释了这种方法与二维平面成像方法的差异和潜在优势。
We have developed a spectral inversion method for three-dimensional tomography of far-red and near-infrared fluorescent proteins in animals. The method was developed in particular to address the steep light absorption transition of hemoglobin from the visible to the far-red occurring around 600 nm. Using an orthotopic mouse model of brain tumors expressing the red-shifted fluorescent protein mCherry, we demonstrate significant improvements in imaging accuracy over single-wavelength whole body reconstructions. Furthermore, we show an improvement in sensitivity of at least an order of magnitude over green fluorescent protein (GFP) for whole body imaging. We discuss how additional sensitivity gains are expected with the use of further red-shifted fluorescent proteins and we explain the differences and potential advantages of this approach over two-dimensional planar imaging methods.