Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation.
Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation.
复制标题
评估果蝇腹侧沟形成过程中 Rho 激酶活性的关键期。
DOI：
10.1002/dvdy.23859
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Minden,JonathanS
中科院分区：
文献类型：
--
作者：
Krajcovic,MelissaM;Minden,JonathanS
背景：果蝇腹侧沟形成（VFF）是胚胎发育过程中的第一个形态发生事件，是上皮折叠的模型。VFF可细分为五种细胞形状变化：顶膜扁平化、顶基核迁移、顶基细胞缩短、随机顶缩和协同顶缩。这些过程通常被认为是由肌球蛋白II的Rho激酶（Rok）激活驱动的，以刺激顶端肌动球蛋白网络的收缩。为了测试Rok及其下游靶点肌球蛋白II在VFF中的作用，定时注射Rok抑制剂Y-27632，进行。结果：在VFF的协同顶端收缩阶段之前注射Y-27632的胚胎能够执行顶基底核迁移和随机顶端收缩，但不能进入协同顶端收缩阶段。在协同顶端收缩期间注射Y-27632的胚胎恢复到随机顶端收缩和协同顶端收缩之间的过渡点。最后，胚胎注射Y-27632后，开始沟侵入能够完成VFF.Conclusions：这些结果表明，在启动一致的顶端收缩阶段的VFF的Rok活动和肌球蛋白II激活的关键时期。发展动力学241：1729-1743，2012年。© 2012 Wiley Periodicals，Inc.
Background:Drosophila ventral furrow formation (VFF), which is the first morphogenetic event during embryo development, serves as a model for epithelial sheet folding. VFF can be subdivided into five cell shape changes: apical membrane flattening, apicobasal nuclear migration, apicobasal cell shortening, random apical constriction, and concerted apical constriction. These processes are generally believed to be driven by Rho kinase (Rok) activation of myosin II to stimulate the constriction of the apical actomyosin network. To test the role of Rok and its downstream target myosin II in VFF, timed injections of the Rok inhibitor, Y‐27632, were performed.Results:Embryos injected with Y‐27632 before the concerted apical constriction phase of VFF were able to execute apicobasal nuclear migration and random apical constriction, but were unable to enter the concerted apical constriction phase. Embryos injected with Y‐27632 during concerted apical constriction reverted to the transition point between random apical constriction and concerted apical constriction. Finally, embryos injected with Y‐27632 upon the initiation of furrow ingression were able to complete VFF.Conclusions:Together these results suggest a critical period for Rok activity and presumably myosin II activation during the initiation of the concerted apical constriction phase of VFF. Developmental Dynamics 241:1729–1743, 2012. © 2012 Wiley Periodicals, Inc.