Histomorphometric and Micro-computed Tomography Analysis of Pulpal Response to Three Different Pulp Capping Materials
Histomorphometric and Micro-computed Tomography Analysis of Pulpal Response to Three Different Pulp Capping Materials
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DOI：
10.1016/j.joen.2010.11.001
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发表时间：
2011-04-01
影响因子：
4.2
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
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作者：
Al-Hezaimi, Khalid;Salameh, Ziad;Tay, Franklin R.
前言：本研究评价了3种盖髓材料对狒狒牙髓的影响。方法：将4只3岁恒河猴的30颗前磨牙随机分为3种盖髓材料。采用开口设计，将3个实验组(氢氧化钙、ProRoot白色矿物骨料、白色硅酸盐水泥)和对照组(不加盖髓材料)进行动物体内随机分组。动物分为4组(n=8颗牙/牙髓盖髓材料)。4个月后处死动物。对回收的标本进行组织形态计量学分析和显微计算机断层扫描。结果：与氢氧化钙盖髓相比，ProRoot白色矿物三氧化物骨料和白色硅酸盐水泥盖髓在暴露牙髓表面有较厚的修复硬组织沉积，但差异无统计学意义(P>；0.05)。所有标本均未发现管状修复性牙本质。各组牙本质通道均有明显缺陷。阴性对照组无修复性硬组织形成。结论：硅酸盐水泥基材料盖髓形成的修复硬组织与氢氧化钙直接盖髓后形成的硬组织在数量(厚度)上不同，但在性质上没有差别。看来，除了那些通过应用碱性盖髓材料为牙髓干细胞致力于成牙本质细胞谱系的隔离外，其他生长因子和转录因子的上调是必要的。(J Endod 2011；37：507-512)
Introduction: This study evaluated the responses of baboon dental pulps after the application of 3 pulp capping materials. Methods: Thirty premolar teeth in four 3-year old baboons were randomly assigned to 1 of 3 pulp capping materials. A split-mouth design was used, and intra-animal side randomization was applied to the 3 experimental groups (calcium hydroxide, ProRoot white mineral trioxide aggregate, white Portland cement) and the control group (no pulp capping material). Animals were divided into 4 groups (n = 8 teeth/pulp capping material). The animals were killed after 4 months. Histomorphometric analysis and micro-computed tomography were performed on the retrieved specimens. Results: Pulps capped with ProRoot white mineral trioxide aggregate and white Portland cement exhibited thicker reparative hard tissue deposition over the exposed pulps when compared with those that were capped with calcium hydroxide (P < .007), but they were not significantly different from one another (P > .05). No tubular reparative dentin could be identified from any of the specimens. Dentin tunnel defects were evident in all groups. There was no reparative hard tissue formation in the negative control group. Conclusions: Reparative hard tissue formation in pulps capped with Portland cement based materials differs only in quantity (thickness) but not in quality from those that were formed after direct pulp capping with calcium hydroxide. It appears that other growth factors and up-regulation of transcription factors are required apart from those sequestered via the application of alkaline pulp capping materials for dental pulp stem cells to commit to the odontoblast lineage. (J Endod 2011;37:507-512)