Hybrid folic acid-conjugated gold nanorods-loaded human serum albumin nanoparticles for simultaneous photothermal and chemotherapeutic therapy
Hybrid folic acid-conjugated gold nanorods-loaded human serum albumin nanoparticles for simultaneous photothermal and chemotherapeutic therapy
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DOI：
10.1016/j.msec.2018.06.002
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发表时间：
2018-10-01
影响因子：
7.9
通讯作者：
Valdez, Miguel A.
中科院分区：
文献类型：
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作者：
Encinas-Basurto, David;Ibarra, Jaime;Valdez, Miguel A.
含有结构和功能纳米成分的混合纳米粒子可能会为患有癌症等疾病的患者带来更高的成功率和更高的生活质量。在本研究中，我们利用脱溶技术制备的多功能人血清白蛋白纳米颗粒（HSA NP’s），将传统药物阿霉素（Dox）与金纳米棒（AuNR）联合用于光热治疗。叶酸（FA）通过碳二亚胺反应酰胺化与HSA NP结合，为HeLa癌细胞提供了更特异性的纳米平台。负载HSA NP的AuNR中Dox的负载效率可达2 μ g Dox/mg HSA。HSA-AuNR-Dox NP经历不同激光功率（1、0.5和0.2 W）的光热加热；在近红外激光照射10分钟后，分别增加16、8和6摄氏度，达到大块颗粒溶液。当HeLa细胞用这种只含有AuNR的多功能纳米平台处理时，癌细胞在没有照射的情况下细胞存活率为96%，在一次照射后细胞存活率为55%。当Dox存在于纳米平台中时，未辐照和辐照纳米平台的存活率分别为60%和24%。这项研究表明，HSA-AuNR-Dox纳米颗粒是一种合适的系统，可以进行协同化疗和光热治疗。
Hybrid nanoparticles containing both structural and functional nanocomponents might result in higher success and increased quality of life for patients suffering a disease such as cancer. In this study, we combine chemotherapy of conventional drug doxorubicin (Dox) with gold nanorods (AuNR) for photothermal therapy using multifunctional human serum albumin nanoparticles (HSA NP's) fabricated via desolvation technique with high efficiency. Folic acid (FA) was conjugated to HSA NP's trough an amidation via carbodiimide reaction for a more specific nanoplataform to HeLa cancer cells. The loading efficiency of Dox into AuNR loaded-HSA NP reached up to 2 mu g Dox/mg HSA. The HSA-AuNR-Dox NP experienced photothermal heating varying laser potency (1, 0.5 and 0.2 W); reaching the bulk particle solution an increment of 16, 8 and 6 degrees C after 10 min of near-IR laser exposure respectively. When HeLa cells were treated with this multifunctional nanoplataform containing only AuNR, cancer cells experienced 96% cell viability without irradiation and 55% cell viability after just one irradiation session. When Dox is present in the nanoplataform, viability were 60% and 24% for non-irradiated and irradiated nanoplataforms, respectively. This study demonstrates that HSA-AuNR-Dox nanoparticles are suitable systems allowing a synergic chemo and phothothermal therapy.