Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels.
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels.
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DOI：
10.1016/j.biomaterials.2015.08.045
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发表时间：
2015-12
期刊：
影响因子：
14
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Yue K;Trujillo-de Santiago G;Alvarez MM;Tamayol A;Annabi N;Khademhosseini A
甲基丙烯酰化明胶（GelMA）水凝胶由于其适宜的生物学特性和可调节的物理特性，已广泛用于各种生物医学应用。三维（3D）GelMA水凝胶由于存在细胞黏附及基质金属蛋白酶响应性肽基序，与天然细胞外基质（ECM）的一些关键特性极为相似，这使得细胞能够在基于GelMA的支架中增殖和扩散。从加工角度来看，GelMA也具有多种用途。它在光照下发生交联，形成具有可调节机械性能的水凝胶，可模拟天然ECM。它还可以使用不同的方法进行微加工，包括微模塑、光掩模、生物打印、自组装和微流体技术，以生成具有可控结构的构建体。通过将GelMA与纳米粒子（如碳纳米管和氧化石墨烯）以及其他聚合物混合，还可以形成混合水凝胶系统，从而形成具有特定生物应用所需综合性能和特性的网络。近期研究表明，基于GelMA的水凝胶在广泛的应用中表现出色，包括骨、软骨、心脏和血管组织工程等。除组织工程外，GelMA水凝胶的其他应用还包括基础单细胞研究、细胞信号传导、药物和基因递送以及生物传感。
Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.