ALTERED CYTOSOL MEMBRANE ENZYME REDISTRIBUTION ON INTERLEUKIN-3 ACTIVATION OF PROTEIN KINASE-C
ALTERED CYTOSOL MEMBRANE ENZYME REDISTRIBUTION ON INTERLEUKIN-3 ACTIVATION OF PROTEIN KINASE-C
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DOI：
10.1038/315235a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
ANDERSON, WB
中科院分区：
文献类型：
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作者：
FARRAR, WL;THOMAS, TP;ANDERSON, WB
白介素 3 (IL-3) 是生长和分化肽家族的成员，统称为集落刺激因子，可调节造血 1-3。 IL-3 已从 WEHI-3B 细胞条件培养基中高度纯化，最近报道了鼠 IL-3 互补 DNA 的分子克隆4,5。 IL-3 似乎可以刺激多种源自小鼠骨髓的集落形成细胞，因此已以多种名称进行研究，包括爆发促进活性 6、肥大细胞生长因子 7、P 细胞刺激因子 8 和多集落刺激因子 3,9。在这里，我们提供的证据表明，IL-3-受体相互作用刺激 FDC-P1 细胞中蛋白激酶 C (PK-C) 从细胞质到质膜的快速、短暂的重新分布。佛波醇肉豆蔻酸酯乙酸酯 (PMA) 在这些 IL-3 依赖性 FDC-P1 细胞中具有类似的作用。我们的数据表明，IL-3 和佛波酯具有对生长和分化至关重要的跨膜信号传导的共同特征。
Interleukin-3 (IL-3) is a member of a family of growth and differentiation peptides, collectively referred to as colony-stimulating factors, which regulate haematopoiesis1–3. IL-3 has been highly purified from medium conditioned by WEHI-3B cells, and recently the molecular cloning of complementary DNA for murine IL-3 has been reported4,5. IL-3 seems to stimulate a wide range of colony-forming cells derived from murine bone marrow and has consequently been studied under a variety of names, including burst-promoting activity6, mast cell growth factor7, P-cell stimulating factor8and multi-colony-stimulating factor3,9. Here we present evidence that IL-3-receptor interaction stimulates the rapid and transient redistribution of protein kinase C (PK-C) from cytosol to plasma membrane in FDC-P1 cells. Phorbol myristate acetate (PMA) is shown to have a similar effect in these IL-3-dependent FDC-P1 cells. Our data suggest that IL-3 and phorbol esters share a common feature of transmembrane signalling crucial for growth and differentiation.