Interphotoreceptor retinoid-binding protein derived peptide can induce experimental autoimmune uveoretinitis in various rat strains.
Interphotoreceptor retinoid-binding protein derived peptide can induce experimental autoimmune uveoretinitis in various rat strains.
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光感受器间视黄醇结合蛋白衍生肽可在各种大鼠品系中诱导实验性自身免疫性葡萄膜视网膜炎。
DOI：
10.3109/02713689409025141
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发表时间：
1994
影响因子：
2
通讯作者：
Hidehiko Matsuda
中科院分区：
文献类型：
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作者：
Y. Sasamoto;Satoshi Kotake;Koji Yoshikawa;B. Wiggert;Igal Gery;Hidehiko Matsuda
实验性自身免疫性葡萄膜视网膜炎(EAU)是由S抗原、视黄醇受体间结合蛋白等视网膜特异性抗原诱导的眼内炎症性疾病模型。本研究旨在检测IRBP及其衍生的一种多肽对不同品系、不同单倍型大鼠的葡萄膜致病性。IRBP免疫可诱导LEW(RT11)、WKAH(RT1k)W/M(RT1k)、LeJ(RT1j)和BUF(RT1b)大鼠不同程度的EAU。IRBP还可在SDJ(RT1u)中诱导低水平的EAU，但在另一种RT1u单倍型品系TO中未检测到疾病。IRBP衍生肽R16(AA 1177-1191)可引起LEW大鼠的重度EAU和WKAH和W/M品系的中度病变。R16免疫可引起75%的LeJ和20%的BUF大鼠眼部炎症，但对SDJ和BUF大鼠均无致病作用。在本研究中，百日咳杆菌注射剂对肽R16诱导的EAU的影响很小，甚至没有影响。这些数据表明，R16肽除了与RT1结合外，还可以与多种RT1分子结合。此外，我们的观察结果支持这样的观点，即IRBP的某些表位在具有不同HLA单倍型的人类中可能是葡萄膜炎原性的。
Experimental autoimmune uveoretinitis (EAU) is an intraocular inflammatory disease model induced by retinal specific antigens such as S-antigen and interphotoreceptor retinoid-binding protein (IRBP). The present study was aimed at testing the uveitogenicity of IRBP and an IRBP-derived peptide in various strains of rats with different RT1 (major histocompatibility complex in rats) haplotypes. Immunization with IRBP induced distinct EAU in LEW (RT1l), WKAH (RT1k) W/M (RT1k), LEJ (RT1j), and BUF (RT1b) rats. IRBP also induced a low grade of EAU in SDJ (RT1u), but no disease was detected in TO rats, another strain of the RT1u haplotype. IRBP-derived peptide R16 (aa 1177-1191) induced severe EAU in LEW rats and moderate disease in the WKAH and W/M strains. Immunization with R16 also induced low levels of inflammation in eyes of 75% and 20% of LEJ and BUF rats, respectively, but this peptide did not cause any disease in SDJ and TO rats. Injection of Bordetella pertussis had minimum or no effect on the induction of EAU by peptide R16 in this study. These data thus indicate that peptide R16 can bind to various RT1 molecules in addition to RT1l. Further, our observations support the notion that certain epitopes of IRBP could be uveitogenic in humans with different HLA haplotypes.
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Donoso,LA;Merryman,CF;Sery,T;Sanders,R;Vrabec,T;Fong,SL
通讯作者：
Fong,SL