Normative levels of Interleukin 16 in umbilical cord blood
Normative levels of Interleukin 16 in umbilical cord blood
复制标题
DOI：
10.1016/j.clinbiochem.2013.07.012
复制
发表时间：
2013-12-01
影响因子：
2.8
通讯作者：
Murray, D. M.
中科院分区：
文献类型：
--
作者：
Denihan, N. M.;Looney, A. M.;Murray, D. M.
目的：对缺氧缺血性脑病（HIE）的早期和准确的预后预测仍然是至关重要的。我们以前已经证明，白细胞介素16（IL-16）是提出了在脐带血（UCB）的婴儿中度和重度HIE，并有可能被开发为一个预测生物标志物。UCB中IL-16的正常参考范围之前尚未描述。本研究的目的是确定正常水平的IL-16在足月新生儿使用脐带血以下简单的分娩和检查脐带IL-16 values.Design和方法的劳动力的影响：足月婴儿被招募作为一个正在进行的出生队列研究的一部分，科克基线出生队列研究。所有人都在出生后3小时内在-80摄氏度下抽取UCB并进行生物银行。从该基于群体的队列研究中选择用于该实验的样品，以代表无并发症的产前剖腹产和自然阴道分娩。使用ELISA Quantikine（R）（R&D Systems，Europe）对血浆EDTA进行分析。结果：分析了来自48名具有两种分娩方式的婴儿的样品：自然阴道分娩（n = 12名男性，n = 12名女性）和选择性剖腹产（n = 12名男性，n = 12名女性）。所有样本的范围均在87.0 - 114.6 pg/ml之间呈正态分布。IL-16的总体平均值（SD）为102.9（21.5）pg/ml。水平不受自发阴道分娩或gender.Conclusion：我们已经描述了第一次在分娩前和分娩后的健康足月婴儿脐带血浆IL-16水平的预期范围。(C)2013年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: The need for early and accurate prediction of outcome in hypoxic-ischaemic encephalopathy (HIE) remains critical. We have previously demonstrated that Interleulkin 16 (IL-16) is raised in the umbilical cord blood (UCB) of infants with moderate and severe HIE and has the potential to be developed as a predictive biomarker. Normal reference ranges for IL-16 in UCB have not been previously described. The aim of this study was to determine normative levels of IL-16 in full term neonates using UCB following uncomplicated deliveries and to examine the effect of labour on cord IL-16 values.Design and methods: Full term infants were recruited as part of an ongoing birth cohort study, the Cork BASELINE Birth Cohort Study. All had UCB drawn and bio-banked at - 80 degrees C, within 3 hours of birth. Samples for this experiment were chosen from this population based cohort study to represent uncomplicated pre-labour caesarean sections and spontaneous vaginal deliveries. Analysis was performed on plasma EDTA, using ELISA Quantikine (R) (R&D Systems, Europe).Results: Samples were analysed from 48 infants with two modes of delivery; spontaneous vaginal delivery (n = 12 male, n = 12 female) and elective caesarean section (n = 12 male, n = 12 female). The range of all samples was normally distributed between 87.0 and 114.6 pg/ml. Overall mean (SD) for IL-16 was 102.9 (21.5) pg/ml. Levels were not affected by spontaneous vaginal delivery or gender.Conclusion: For the first time we have described the expected range of cord plasma IL-16 levels in healthy term infants following pre-labour and post-labour delivery. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.