Metastatic effect of LY-6K gene in breast cancer cells
Metastatic effect of LY-6K gene in breast cancer cells
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DOI：
10.3892/ijo_00000371
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发表时间：
2009-09-01
影响因子：
5.2
通讯作者：
Park, Jong Hoon
中科院分区：
文献类型：
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作者：
Choi, Suh Hee;Kong, Hyun Kyung;Park, Jong Hoon
乳腺癌细胞中尿激酶受体的过度表达导致肿瘤侵袭、生长和转移增加。由于LY-6K属于Ly6/uPAR超家族，我们评估了LY-6K与乳腺癌细胞系转移的关系以及LY-6K基因如何介导癌细胞转移。通过转染LY-6K裸DNA并处理LY-6K siRNA来研究LY-6K基因表达上调或下调的细胞的迁移能力。进行伤口愈合、迁移和侵袭的测定。检测结果表明，当乳腺癌细胞中LY-6K基因过度表达或LY-6K蛋白异常表达时，细胞迁移显着增加。此外，LY6K引起癌细胞转移，并且与Ras/ERK信号通路有关。我们的结果表明，LY-6K 通过 Ras/ERK 通路影响细胞迁移和侵袭，有意义地参与乳腺癌细胞转移。
The over-expression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis. As LY-6K belongs to the Ly6/uPAR superfamily, we assessed the association of LY-6K with metastasis in breast cancer cell lines and how the LY-6K gene mediates cancer cell metastasis. The migration ability of cells in which expression of LY-6K gene is up- or downregulated was investigated by transfecting LY-6K naked DNA and treating LY-6K siRNA. Assays of wound healing, migration and invasion were performed. The assays demonstrated that cell migration significantly increased when LY-6K gene is over-expressed or LY-6K protein is abnormally expressed in breast cancer cells. In addition, LY6K caused cancer cell metastasis and it is linked to the Ras/ERK signaling pathway. Our results suggest that LY-6K meaningfully participates in breast cancer cell metastasis by influencing cell migration and invasion through the Ras/ERK pathway.