Activin promotes oocyte development in ovine preantral follicles in vitro.
Activin promotes oocyte development in ovine preantral follicles in vitro.
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DOI：
10.1186/1477-7827-1-76
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发表时间：
2003-11-03
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影响因子：
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通讯作者：
Telfer, Evelyn E
中科院分区：
文献类型：
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作者：
Thomas, Fiona H;Armstrong, David G;Telfer, Evelyn E
激活素被认为是许多生殖过程的重要调节因子。本研究旨在探讨激活素A对绵羊腔前卵泡体外发育的影响。将机械分离的腔前卵泡（161 +/- 2 μ m）在人重组激活素A（0、10和100 ng/ml）存在下培养6天。每隔一天更换一半培养基，并测量卵泡直径。随后使用延迟增强镧系元素荧光免疫测定法（DELFIA）分析条件培养基的雌二醇含量。在培养期结束时，固定卵泡并进行组织学处理，之后测量卵母细胞直径和颗粒细胞死亡。在所有组中，在6天内有显著的卵泡生长（p < 0.001）。到第6天，两种浓度的激活素增加卵泡生长超过对照水平（p < 0.05）。在所有组中，到培养第6天卵母细胞直径也显著增加（p < 0.05），100 ng/ml激活素使卵母细胞直径增加超过对照水平（p < 0.05）。在两种浓度下，激活素在培养的第2天增加雌二醇的产生，但这种增加在培养期间没有持续。此外，激活素对窦形成或卵泡存活没有任何影响。总之，激活素促进绵羊腔前卵泡和卵母细胞的体外生长，但没有加速卵泡分化超过6天的培养期。这些结果支持激活素A在早期卵母细胞和卵泡发育中的旁分泌作用。
Activins have been implicated as important regulating factors for many reproductive processes. The aim of this study was to determine the effect of activin A on the development of ovine preantral follicles in vitro. Mechanically isolated preantral follicles (161 +/- 2 microm) were cultured for 6 days in the presence of human recombinant activin A (0, 10 and 100 ng/ml). Half of the medium was replaced every second day and follicle diameters were measured. Conditioned medium was subsequently analysed for oestradiol content using a delayed enhancement lanthanide fluorometric immunoassay (DELFIA). At the end of the culture period, follicles were fixed and processed for histology, after which oocyte diameter and granulosa cell death were measured. There was significant follicle growth over 6 days in all groups (p < 0.001). Activin, at both concentrations, increased follicle growth over control levels by Day 6 (p < 0.05). Oocyte diameters were also significantly increased by Day 6 of culture in all groups (p < 0.05), with 100 ng/ml activin increasing oocyte diameter over control levels (p < 0.05). Activin, at both concentrations, increased oestradiol production on Day 2 of culture, but this increase was not sustained during the culture period. Moreover, activin did not have any effect on antrum formation or follicle survival. In conclusion, activin promoted ovine preantral follicle and oocyte growth in vitro, but did not accelerate follicle differentiation over a six-day culture period. These results support a paracrine role for activin A during early oocyte and follicular development.