The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus
The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus
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DOI：
10.1111/j.1574-6968.2012.02502.x
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发表时间：
2012-04-01
影响因子：
2.1
通讯作者：
Foster, Simon J.
中科院分区：
文献类型：
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作者：
Hurd, Alexander F.;Garcia-Lara, Jorge;Foster, Simon J.
金黄色葡萄球菌的铁调节表面决定蛋白（Isd）在铁限制期间表达，并被认为参与从血红素中清除铁。在这项研究中，编码表面蛋白 IsdA、IsdB 和 IsdH 的基因被灭活，以确定它们的组合作用。发现三重突变体在任何铁限制测试条件下都没有生长缺陷。还使用类似于金黄色葡萄球菌全身性疾病的小鼠化脓性关节炎模型，与其他同基因亲本相比，没有观察到三重突变体的细菌载量有显着差异。
The iron-regulated surface determinant proteins (Isd) of Staphylococcus aureus are expressed during iron limitation and have been proposed to be involved in the scavenging of iron from heme. In this study, the genes encoding the surface proteins IsdA, IsdB, and IsdH were inactivated in order to determine their combined role. The triple mutant was found to have no defect in growth under any conditions of iron limitation tested. Also using a mouse septic arthritis model of S.similar to aureus systemic disease, no significant difference in bacterial load was observed for the triple mutant, compared with its otherwise isogenic parent.