The viscoelastic behaviors of several kinds of cancer cells and normal cells
The viscoelastic behaviors of several kinds of cancer cells and normal cells
复制标题
几种癌细胞和正常细胞的粘弹性行为
DOI：
10.1016/j.jmbbm.2018.11.029
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发表时间：
2019
影响因子：
3.9
通讯作者：
Wang Guohui
中科院分区：
文献类型：
--
作者：
Xie Yongfang;Wang Mingling;Cheng Min;Gao Zhiqin;Wang Guohui
本研究的目的是利用微管抽吸技术结合标准线性粘弹性固体模型来研究癌细胞和正常细胞的粘弹性行为。采用微管抽吸技术检测了人皮肤细胞和人皮肤癌细胞、人胎儿肺成纤维细胞和人肺癌细胞、人乳腺成纤维细胞和人乳腺癌细胞、人肝细胞和人肝细胞肝癌细胞对细胞系的粘弹性行为。采用开尔文标准线性粘弹性固体模型计算了细胞粘弹性参数（瞬时模量use0、与长期平衡me∞相关的平衡模量use0和表观粘弹性μ）。结果表明，肿瘤细胞更易变形，肿瘤细胞的粘弹性参数（E0,E∞,μ）明显低于正常细胞（P < 0.0001）。正常细胞间粘弹性参数（E0、E∞、μ）差异有统计学意义（P < 0.05），不同肿瘤细胞间粘弹性参数差异无统计学意义（P > 0.05）。这些发现可能对癌细胞的鉴别和诊断有一定的指导意义，并为解释癌细胞发生机制和癌症治疗提供依据。
The purpose of this study was to investigate the viscoelastic behaviors of cancer cells and normal cells using the micropipette aspiration technique combined with the standard linear viscoelastic solid model. The viscoelastic behaviors of pairs of cell lines (human skin cells and human skin cancer cells, human fetal lung fibroblasts and human lung cancer cells, human mammary fibroblasts and human breast cancer cells, and human hepatocyte cells and human hepatocellular carcinoma cells) were tested by the micropipette aspiration technique. The cellular viscoelastic parameters (the instantaneous modulusE0, the equilibrium modulus associated with long term equilibriumE∞, and the apparent viscosityμ) were calculated using a Kelvin standard linear viscoelastic solid model. The present results indicate that the cancer cells were easier to deform, and the viscoelastic parameters (E0,E∞,μ) of the cancer cells were significantly lower than their corresponding normal cells (P < 0.0001). The viscoelastic parameters (E0,E∞,μ) among some normal cells showed significant differences (P < 0.05), while the different cancer cells showed no significant differences (P > 0.05). These findings may be relevant for the identification and diagnosis of cancer cells as well as providing an explanation of this occurrence mechanism in cancer cells and cancer treatment.