Identification of an Apoplastic Protein Involved in the Initial Phase of Salt Stress Response in Rice Root by Two-Dimensional Electrophoresis
Identification of an Apoplastic Protein Involved in the Initial Phase of Salt Stress Response in Rice Root by Two-Dimensional Electrophoresis
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二维电泳鉴定参与水稻根盐胁迫反应初始阶段的质外体蛋白
DOI：
10.1104/pp.108.131144
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发表时间：
2009-02-01
期刊：
影响因子：
7.4
通讯作者：
Guo, Yi
中科院分区：
文献类型：
--
作者：
Zhang, Lei;Tian, Li-Hong;Guo, Yi
植物细胞外质体不仅是环境与原生质体之间的屏障，而且是连接环境与原生质体之间的纽带，在植物的代谢和信号传递中发挥着多种功能。为了研究外质体蛋白在盐胁迫响应中的作用，将10 d龄水稻（Oryza sativa）植株用200 mM NaCl处理1、3和6 h，提取可溶性外质体蛋白，并通过双向电泳与未处理对照进行差异分析。质谱法鉴定了10个丰度显著增加或减少的蛋白点。这些蛋白质包括一些众所周知的生物和非生物应激相关蛋白质。其中，具有胞外结构域样富半胱氨酸基序（DUF26）的外质体蛋白O. sativa root meander curling （OsRMC）在盐胁迫的初始阶段丰度急剧增加。为了评估OsRMC在盐胁迫响应中的功能，我们制备了OsRMC RNA干扰转基因水稻。结果表明，与非转基因水稻相比，敲低OsRMC的表达水平导致转基因水稻种子萌发不敏感，生长抑制增强，耐盐性提高。这些结果表明，植物外胞体蛋白可能在植物盐胁迫反应中起重要作用。
The apoplast of plant cells, which carries out multiple functions in plant metabolism and signaling, is not only a barrier but also the linker between the environment and the protoplast. To investigate the role of apoplastic proteins in the salt stress response, 10-d-old rice (Oryza sativa) plants were treated with 200 mM NaCl for 1, 3, or 6 h, and the soluble apoplast proteins were extracted for differential analysis compared with untreated controls using two-dimensional electrophoresis. Ten protein spots that increased or decreased significantly in abundance were identified by mass spectrometry. These proteins included some well-known biotic and abiotic stress-related proteins. Among them, an apoplastic protein, with extracellular domain-like cysteine-rich motifs (DUF26), O. sativa root meander curling (OsRMC), has shown drastically increased abundance in response to salt stress during the initial phase. OsRMC RNA interference transgenic rice has been generated to assess the function of OsRMC in the salt stress response. The results show that knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition, and improved salt stress tolerance to NaCl than in untransgenic plants. These results indicate that plant apoplastic proteins may have important roles in the plant salt stress response.