Relation of oxidative protein damage and nitrotyrosine levels in the aging rat brain
Relation of oxidative protein damage and nitrotyrosine levels in the aging rat brain
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DOI：
10.1016/s0531-5565(00)00197-2
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发表时间：
2001-02-01
影响因子：
3.9
通讯作者：
Sivas, A
中科院分区：
文献类型：
--
作者：
Çakatay, U;Telci, A;Sivas, A
氧化应激的增加可能促进了衰老大鼠脑内氧化蛋白损伤的发展。在本研究中，我们研究了幼年、成年和老年Wister大鼠脑组织中硝基酪氨酸水平与其他氧化蛋白质损伤参数(如蛋白质羰基和蛋白质硫醇)以及氧化应激参数(如总硫醇、非蛋白质硫醇和过氧化脂质)之间的关系。老年大鼠脑内硝基酪氨酸水平明显低于青年大鼠。青年大鼠和成年大鼠在这些参数上没有显著差异，但老年大鼠的脑蛋白质羰基和脂质过氧化氢水平显著高于青年大鼠和成年大鼠。另一方面，老年大鼠脑组织总硫醇、非蛋白硫醇和蛋白硫醇水平显著低于青年和成年大鼠。我们发现蛋白质羰基和脂质过氧化氢水平之间的强烈相关性表明，在老化的脑组织中，蛋白质氧化和脂质过氧化之间存在显着的关系。这项研究的结果表明，蛋白质羰基形成既是脑老化的敏感标志，也是脑老化的特异性标志。然而，老年大鼠硝基酪氨酸水平的下降与预期相反，可能是由于衰老大鼠大脑中氧化蛋白损伤以外的机制。(C)2001 Elsevier Science Inc.保留所有权利。
An increase in oxidative stress may contribute to the development of oxidative protein damage in the aging rat brain. In the present study, we investigated the relation between nitrotyrosine levels and other oxidative protein damage parameters such as protein carbonyl and protein thiol, as well as oxidative stress parameters such as total thiol, nonprotein thiol, and lipid hydroperoxides in the brain tissue of young, adult, and old Wister rats. Brain nitrotyrosine levels of old rats were significantly decreased compared with those of young rats. Young and adult rats were not significantly different as far as these parameters were concerned, however, brain protein carbonyl and lipid hydroperoxide levels of old rats were significantly increased compared with those of young and adult rats. On the other hand, brain tissue total thiol, nonprotein thiol, and protein thiol levels of old rats were significantly decreased compared with those of young and adult rats. The strong correlation we found between protein carbonyl and lipid hydroperoxide levels indicates a striking relation between protein oxidation and lipid peroxidation in the aging brain tissue. The results of this study suggest that protein carbonyl formation is both a sensitive and a specific marker of brain aging. However, decreased nitrotyrosine levels in old rats, in contradiction to the expected, may be due to mechanisms other than oxidative protein damage in the aging rat brain. (C) 2001 Elsevier Science Inc. All rights reserved.