The phenotypic response of cultured quail trunk neural crest cells to a reconstituted basement membrane-like matrix is specific.
The phenotypic response of cultured quail trunk neural crest cells to a reconstituted basement membrane-like matrix is specific.
复制标题
培养的鹌鹑躯干神经嵴细胞对重建的基底膜样基质的表型反应是特异性的。
DOI：
10.1016/0012-1606(90)90118-3
复制
发表时间：
1990
影响因子：
2.7
通讯作者：
Forbes,ME
中科院分区：
文献类型：
--
作者：
Maxwell,GD;Forbes,ME
以前的工作已经证明，一个重建的基底膜（RBM）样基质刺激发育的神经嵴文化中的儿茶酚胺（CA）含细胞。在目前的工作中，我们发现，酪氨酸羟化酶和生长抑素免疫反应细胞的比例大幅增加的覆盖RBM矩阵。与此相反，有很少或没有刺激的细胞具有其他几个表型标记，包括A2 B5，E/C8，血管活性肠多肽，和低和中分子量的禽神经丝蛋白的发展。这些结果表明，反应的神经嵴细胞的RBM矩阵是特定的一小部分表型。此外，我们证明，在RBM凝胶覆盖的存在下发展的肾上腺素能细胞的表型是非常相似的，如果不是相同的，在RBM凝胶的情况下分化的肾上腺素能细胞。
Previous work has demonstrated that a reconstituted basement membrane (RBM)-like matrix stimulates the development of catecholamine (CA)-containing cells in neural crest cultures. In the present work, we found that the proportion of tyrosine hydroxylase and somatostatin immunoreactive cells was increased substantially by an overlay of the RBM matrix. In contrast, there was little or no stimulation of the development of cells possessing several other phenotypic markers including A2B5, E/C8, vasoactive intestinal polypeptide, and the low and middle molecular weight avian neurofilament proteins. These results demonstrate that the response of neural crest cells to the RBM matrix is specific to a small set of phenotypes. In addition, we demonstrate that the phenotype of the adrenergic cells which develop in the presence of the RBM gel overlay is very similar, if not identical, to that of the adrenergic cells which differentiate in the absence of the RBM gel.