Curcumin-crosslinked acellular bovine pericardium for the application of calcification inhibition heart valves
Curcumin-crosslinked acellular bovine pericardium for the application of calcification inhibition heart valves
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姜黄素交联脱细胞牛心包在钙化抑制心脏瓣膜中的应用
DOI：
10.1088/1748-605x/ab6f46
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发表时间：
2020-07-01
影响因子：
4
通讯作者：
Wang Zhihong
中科院分区：
文献类型：
--
作者：
Liu Jing;Li Binhan;Wang Zhihong
戊二醛(GA)交联的牛或猪心包组织在构建生物瓣膜时表现出高的细胞毒性和钙化，这加速了瓣叶的破坏，并促使人们探索替代方案。姜黄素、原花青素和槲皮素等多酚类物质在胶原蛋白和弹性蛋白的交联型支架中显示出很强的钙化抑制潜力。在这里，我们开发了一种创新的酚醛固定技术，将姜黄素作为瓣膜材料的交联剂。X射线光电子能谱和傅里叶变换红外光谱分析证实了姜黄素与去细胞牛心包之间存在氢键。重要的是，通过体外实验、幼年大鼠皮下植入模型和成骨分化模型实验，证明了姜黄素交联牛心包的钙化抑制能力。此外，与GA组和商品化产品相比，姜黄素固定的牛心包在机械性能、血液相容性和细胞相容性方面表现出更好的性能。综上所述，我们证明了姜黄素是一种可行的固定无细胞牛心包的交联剂，在生物医学方面显示出巨大的应用潜力，特别是在具有钙化抑制能力的心血管生物材料中。
Glutaraldehyde (GA) crosslinked bovine or porcine pericardium tissues exhibit high cell toxicity and calcification in the construction of bioprosthetic valves, which accelerate the failure of valve leaflets and motivate the exploration for alternatives. Polyphenols, including curcumin, procyanidin and quercetin, etc, have showed great calcification inhibition potential in crosslinking collagen and elastin scaffolds. Herein, we developed an innovative phenolic fixing technique by using curcumin as the crosslinking reagent for valvular materials. X-ray photoelectron spectroscopy and Fourier transform infrared spectrometry assessments confirmed the hydrogen bond between curcumin and acellular bovine pericardium. Importantly, the calcification inhibition capability of the curcumin-crosslinked bovine pericardium was proved by the dramatically reduced Ca2+ content in the curcumin-fixed group in in vitro assay, a juvenile rat subcutaneous implants model, as well as an osteogenic differentiation model. In addition, the results showed that the curcumin-fixed bovine pericardium exhibited better performance in the areas of mechanical performance, hemocompatibility and cytocompatibility, in comparison with the GA group and the commercialized product. In summary, we demonstrated that curcumin was a feasible crosslinking reagent to fix acellular bovine pericardium, which showed great potential for biomedical applications, particularly in cardiovascular biomaterials with calcification inhibition capacity.