A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly
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DOI：
10.1016/s0092-8674(00)81365-3
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发表时间：
1996-11-01
期刊：
影响因子：
64.5
通讯作者：
Cleveland, DW
中科院分区：
文献类型：
--
作者：
Merdes, A;Ramyar, K;Cleveland, DW
NuMA在间期是一种核蛋白，但在有丝分裂早期重新分布到纺锤体两极。为了研究其在纺锤体形成过程中的作用，我们测试了蛙卵提取物中的纺锤体组装，其中NuMA被免疫耗竭。免疫耗竭显示NuMA与细胞质动力蛋白和动力蛋白形成复合物。耗尽提取物未能组装正常的有丝分裂纺锤体，生产，相反，染色质相关的不规则阵列的微管缺乏特征纺锤体极。NuMA尾部的一个亚结构域通过介导微管成束诱导微管星形细胞形成。我们的研究结果表明，NuMA与细胞质动力蛋白和动力蛋白形成双功能复合物，可以将微管拴在纺锤体极点，这对有丝分裂纺锤体极点组装和稳定至关重要。
NuMA is a nuclear protein during interphase but redistributes to the spindle poles early in mitosis. To investigate its role during spindle formation, we tested spindle assembly in frog egg extracts from which NuMA was immunodepleted. Immunodepletion revealed that NuMA forms a complex with cytoplasmic dynein and dynactin. The depleted extracts failed to assemble normal mitotic spindles, producing, instead, chromatin-associated irregular arrays of microtubules lacking characteristic spindle poles. A subdomain of the NuMA tail was shown to induce microtubule aster formation by mediating microtubule bundling. Our findings suggest that NuMA forms bifunctional complexes with cytoplasmic dynein and dynactin that can tether microtubules at the spindle poles and that are essential for mitotic spindle pole assembly and stabilization.