Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development
Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development
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DOI：
10.1016/j.plaphy.2008.12.004
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发表时间：
2009-04-01
影响因子：
6.5
通讯作者：
Geny, Laurence
中科院分区：
文献类型：
--
作者：
Gagne, Severine;Lacampagne, Soizic;Geny, Laurence
原花青素，或缩合单宁，是对葡萄和葡萄酒质量至关重要的多酚化合物。近年来，对其主要亚基(+)-儿茶素和(-)-表儿茶素的生物合成的研究取得了重大进展，它们分别是由亮氨酸花青素还原酶(LAR)和花青素还原酶(ANR)催化产生的。表达研究已经发表，但没有关于酶活性的数据。在我们的工作中，我们设计了检测LAR和ANR活性的方法，并确定了它们在葡萄花、幼果和果皮生长过程中的发育。赤霞珠-苏维翁。我们还研究了化合物在这些组织中的积累，并重点研究了结构基因和参与调控它们的转录因子VvMYB5a和VvMYBPA1的表达。生物合成基因很早就表达，LAR和ANR在开花和浆果生长初期以及果皮变色期间都已经活跃。我们测定的图谱与总单宁、儿茶素和表儿茶素浓度相关。证实了VvMYB5a和VvMYBPA1的参与，并建立了VvLAR转录本的特异性表达模式。(C)2008年爱思唯尔·马森公司。版权所有。
Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (-)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively. Expression studies had been published but no data were available on enzyme activity. In our work, we devised assays to measure LAR and ANR activity and determine their development throughout the growth of flowers, young berries, and skins of Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them: VvMYB5a and VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of VvMYB5a and VvMYBPA1 was confirmed and specific expression patterns were also established for VvLAR transcripts. (C) 2008 Elsevier Masson SAS. All rights reserved.