The yeast ADP/ATP carrier. Mutagenesis and second-site revertants.
The yeast ADP/ATP carrier. Mutagenesis and second-site revertants.
复制标题
酵母 ADP/ATP 载体。
DOI：
10.1016/0005-2728(96)00063-1
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Nelson,DR
中科院分区：
文献类型：
--
作者：
Nelson,DR
本文报道了酵母ADP/ATP载体AAC 2的自发第二位点回复突变体的诱变和筛选结果。目前，已经在35个地点在AAC 2中产生了50个突变体。在K38、K48、R96、D149、R152、R204、D249、R252、R253、R254和R294处携带突变的酵母都不能在甘油上生长。这些突变体中有七个产生了第二个网站的回复突变体时，丰富的酵母培养基中含有甘油和乙醇平板。R96突变体以及R254和R253突变体在AAC 2分子中产生类似的变化，因为相同的位点受到其回复突变体突变的影响。这种突变和回复突变体系统现在准备产生对ADP和ATP运输动力学以及线粒体载体结构的一般见解。
Results of mutagenesis and selection of spontaneous second-site revertants of the yeast ADP/ATP carrier AAC2 is described. Currently, 50 mutants have been made in AAC2 at 35 locations. Yeast carrying mutations at K38, K48, R96, D149, R152, R204, D249, R252, R253, R254 and R294 are all unable to grow on glycerol. Seven of these mutants have yielded second-site revertants when plated on rich yeast media containing glycerol and ethanol. The R96 mutants and the R254 and R253 mutants produce similar changes in the AAC2 molecule because the same sites are affected by their revertant mutations. This system of mutations and revertants is now poised to yield insights into the dynamics of ADP and ATP transport, and mitochondrial carrier structure in general.