Detection of alleles associated with resistance to chemical insecticide in the malaria vector Anopheles arabiensis in Santiago, Cabo Verde
Detection of alleles associated with resistance to chemical insecticide in the malaria vector Anopheles arabiensis in Santiago, Cabo Verde
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DOI：
10.1186/s12936-019-2757-3
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发表时间：
2019-04-05
期刊：
影响因子：
3
通讯作者：
Junqueira Ayres, Constancia Flavia
中科院分区：
文献类型：
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作者：
da Cruz, Derciliano Lopes;Santos Paiva, Marcelo Henrique;Junqueira Ayres, Constancia Flavia
背景：冈比亚按蚊（Anopheles gambiae）是世界范围内主要的疟疾传播媒介。由于缺乏预防疟疾的疫苗，减少这一疾病影响的主要方法依赖于使用化学杀虫剂来控制其病媒。然而，这类化合物的大量使用已导致非洲若干按蚊种群出现抗药性。这项研究的目的是调查来自佛得角群岛首府普拉亚市的阿拉伯按蚊种群中抗性等位基因的存在，佛得角群岛是世界卫生组织正在消除疟疾当地传播的国家名单上的国家之一。方法：在普拉亚市3个地区用一品脱斗采集按蚊属幼虫。对幼虫进行喂养和饲养，直到成蚊出现，并对这些成蚊进行形态鉴定。此外，利用IGS标记和所有的An进行分子鉴定。对arabiensis样本进行PCR筛选，以筛选Ace-1、Na-v和GSTE2基因中与抗性相关的突变。结果：捕获蚊虫440只，形态鉴定为按蚊的占52.3%；感冈比亚蚊（33.1%）和比托利按蚊（46.7%）。分子鉴定结果表明，安。冈比亚s.l.a。arabiensis。筛选到Ace-1基因突变G119S和GSTE2基因突变L119F，但未在所有样本中发现。然而，测序分析显示，GSTE2存在37个单倍型，16个多态性位点，遗传多样性高（pi = 2.67）。Na-v（电压门控钠通道基因）L1014S突变检出率为7.3%。结论：本研究首次对安家鼠杀虫剂抗性等位基因的循环进行了调查。产自佛得角的阿拉伯树。安人L1014S等位基因的循环。普拉亚市的arabiensis表明，拟除虫菊酯类杀虫剂耐药性可能会出现，很快就会被选择，并可能影响佛得角消除疟疾的进程。应继续进行耐药性分子监测，以指导研究地区病媒控制策略的制定。
Background: Mosquitoes of the Anopheles gambiae complex are the main malaria vectors worldwide. Due to the lack of a vaccine to prevent malaria, the principal way to reduce the impact of this disease relies on the use of chemical insecticides to control its vectors. However, the intensive use of such compounds has led to the emergence of insecticide resistance in several Anopheles populations in Africa. This study aimed to investigate the presence of resistance alleles in an Anopheles arabiensis population from the City of Praia, capital of the Archipelago Cabo Verde, one of the countries on the World Health Organization list of countries that are on a path to eliminate local transmission of malaria.Methods: Larvae from the Anopheles genus were collected using a one-pint dipper in three areas of City of Praia. Larvae were fed and maintained until the emergence of adult mosquitoes, and these were morphologically identified. In addition, molecular identification was performed using IGS markers and all An. arabiensis samples were subjected to PCR to screen for mutations associated to resistance in the Ace-1, Na-v and GSTE2 genes.Results: From a total of 440 mosquitoes collected, 52.3% were morphologically identified as An. gambiae sensu lato (s.l.) and 46.7% as Anopheles pretoriensis. The molecular identification showed that 100% of the An. gambiae s.l. were An. arabiensis. The mutations G119S in the Ace-1 gene and L119F in the GSTE2 gene were screened but not found in any sample. However, sequencing analysis for GSTE2 revealed the presence of 37 haplotypes, 16 polymorphic sites and a high genetic diversity (pi = 2.67). The L1014S mutation in the Na-v (voltage-gated sodium channel gene) was detected at a frequency of 7.3%.Conclusion: This is the first study to investigate the circulation of insecticide resistance alleles in An. arabiensis from Cabo Verde. The circulation of the L1014S allele in the population of An. arabiensis in the city of Praia suggests that pyrethroid resistance may arise, be quickly selected, and may affect the process of malaria elimination in Cabo Verde. Molecular monitoring of resistance should continue in order to guide the development of strategies to be used in vector control in the study region.