Proliferation rate of rabbit corneal epithelium during overnight rigid contact lens wear.
Proliferation rate of rabbit corneal epithelium during overnight rigid contact lens wear.
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发表时间：
2001-11
影响因子：
4.4
通讯作者：
P. Ladage;K. Yamamoto;D. H. Ren;L. Li;J. Jester;W. Petroll;J. Bergmanson;H. Cavanagh
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文献类型：
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作者：
P. Ladage;K. Yamamoto;D. H. Ren;L. Li;J. Jester;W. Petroll;J. Bergmanson;H. Cavanagh
目的观察正常角膜上皮和长期硬性透气性透镜配戴过程中角膜上皮细胞的增殖情况。方法新西兰白色兔23只，单侧配戴低氧透过率（Dk/t）或高Dk/tRGP透镜，另一侧眼作为对照。24小时后注射5-溴-2-脱氧尿苷（BrdU），并在注射后1、3、7天3个时间点处死。对角膜进行免疫细胞化学处理，并用落射荧光显微镜从上级利姆布斯到中央上皮拍摄连续数字图像。定量BrdU标记的细胞对的总数。结果正常角膜利姆布斯的BrdU标记细胞数明显少于中央上皮和周边上皮（P < 0.05）。邻近利姆布斯的周围上皮细胞标记细胞数最多（P < 0.05）。两种类型的RGP晶状体均使利姆布斯中BrdU标记增加，中央上皮中BrdU标记显著减少（低Dk/t为80%，高Dk/t为37%）。在注射BrdU后第3天和第7天，低Dk/t透镜继续显示BrdU标记减少，而利姆布斯仍然增加。然而，与对照角膜相比，Hyper-Dk/t透镜配戴显示持续的角膜缘升高，但在第3天和第7天中心BrdU标记细胞的数量相等。在RGP透镜配戴期间，角膜细胞意外地显示BrdU标记。结论：正常兔角膜中，利姆布斯、周边和中央上皮的增殖率不同。RGP透镜磨损显著改变了上皮细胞的稳态增殖模式，其中低Dk/t透镜具有最显著的效果。RGP接触透镜配戴似乎刺激角膜细胞增殖。
PURPOSE To examine cell proliferation of the normal corneal epithelium and during extended rigid gas-permeable (RGP) lens wear. METHODS Twenty-three New Zealand White rabbits were fitted unilaterally with either a low oxygen transmissible (Dk/t) or hyper-Dk/t RGP lens, with the other eye serving as a control. The rabbits were injected with 5-bromo-2-deoxyuridine (BrdU) 24-hours later and killed at three time points: 1, 3, and 7 days after injection. Corneas were processed for immunocytochemistry, and sequential digital images were taken from the superior limbus to the central epithelium with an epifluorescence microscope. The total number of BrdU-labeled cell pairs was quantified. RESULTS The limbus in normal corneas was significantly less populated with BrdU-labeled cells than the central and peripheral epithelium (P < 0.05). The peripheral epithelium adjacent to the limbus was marked by a peak of labeled cells (P < 0.05). Both types of RGP lenses produced an increase in BrdU labeling in the limbus and a dramatic decrease in the central epithelium (80% for low Dk/t, 37% for hyper Dk/t). At day 3 and 7 after BrdU injection, the low-Dk/t lens continued to show decreased BrdU labeling centrally, whereas the limbus remained increased. Hyper-Dk/t lens wear however, showed persistent limbal elevation but equivalent numbers of BrdU-labeled cells centrally at days 3 and 7, compared with control corneas. Keratocytes unexpectedly showed BrdU labeling during RGP lens wear. CONCLUSIONS Limbus, peripheral, and central epithelium were characterized by different proliferation rates in the normal rabbit cornea. RGP lens wear significantly altered the homeostatic proliferation pattern of the epithelium with the low-Dk/t lens having the most dramatic effect. RGP contact lens wear appears to stimulate proliferation of keratocytes.