Complement Fixation in Tuberculosis with the “Partial Antigens” of Deyke and Much
Complement Fixation in Tuberculosis with the “Partial Antigens” of Deyke and Much
复制标题
Deyke 等人的“部分抗原”对结核病的补体固定
作者：
A. Woods;G. E. Bushnell;Cabell Maddux
从结核杆菌中制备了部分抗原，方法是将结核杆菌在1%的乳酸中分解，过滤，并用酒精在不同温度下和乙醚提取。由此产生的抗原似乎主要由各种脂肪酸和结核杆菌的中性脂肪以及微量蛋白质组成。
Partial antigens have been prepared from the tubercle bacillus, by disintegrating the bacilli in 1 per cent lactic acid, filtering, and extracting with alcohol, at varying temperatures, and with ether. The resulting antigens appear to be composed chiefly of the various fatty acids, and neutral fat of the tubercle bacillus together with traces of protein.
One hundred and seventy-six normal and tuberculous sera were titrated against these partial antigens, and against whole bacillus emulsion antigen. Seventy-two of the sera obtained from clinically normal cases, and 91 per cent of the sera from cases classed clinically as inactive tuberculosis reacted negatively to these antigens. Ninety per cent of the sera from cases classed clinically as incipient tuberculosis, 87 per cent of the sera from cases classed as active tuberculosis, and 92 per cent of the sera from advanced tuberculous cases reacted positively to the partial antigens.
Sufficient syphilitic sera could not be obtained to make any determination of the proportion of syphilitic sera which would react positively to these antigens but judging from 27 positive Wassermanns obtained at the same time the sera were examined, there appears to be little relationship between the Wassermann reaction and the reaction to the partial antigens.