Effect of time and dose of indomethacin on follicular prostaglandins and ovulation in the rabbit.
Effect of time and dose of indomethacin on follicular prostaglandins and ovulation in the rabbit.
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DOI：
10.1210/endo-119-2-746
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发表时间：
1986-08
期刊：
影响因子：
4.8
通讯作者：
L. Espey;C. Norris;D. Saphire
中科院分区：
文献类型：
--
作者：
L. Espey;C. Norris;D. Saphire
这项研究特别确定了排卵期间卵巢前列腺素（PGs）何时增加，以及不同剂量的吲哚美辛如何有效地抑制PGs和排卵。在用hCG（50 IU/kg）刺激动物后，每隔一小时取出兔卵泡。将卵泡在0.1 M醋酸盐缓冲液（pH 4.5）中匀浆，并通过RIA测定提取物中的PGE和PGF。通过计算hCG刺激后破裂的成熟卵泡的百分比来确定排卵率。hCG前，PGE和PGF的正常水平分别为111.1 +/- 14.7和51.0 +/- 6.6 pg/mg卵泡。hCG处理后2 h，PGE和PGF分别增加至162.6 +/- 17.0和80.6 +/- 13.3 pg/mg卵泡。大约5小时后，两种PG均出现第二次更剧烈的增加，分别在hCG处理后10小时（即预期排卵时间）达到峰值652.6 +/- 63.5和345.4 +/- 32.3 pg/mg卵泡。我们发现，无论吲哚美辛是早或晚在排卵过程中，这种药物显着减少卵泡PG生产后5分钟内，其管理。例如，仅在hCG后8小时给予10 mg/kg吲哚美辛后5分钟，PGE和PGF分别从317.7 +/- 50.0和125.0 +/- 10.5 pg/mg卵泡降至93.3 +/- 17.4和49.3 +/- 10.5 pg/mg卵泡。出乎意料的是，当给予梯度剂量的吲哚美辛后1或8小时，卵泡PG水平和排卵率之间没有统计学意义的相关性。例如，当在hCG后8小时给予1.25、2.5、5.0和10.0 mg/kg吲哚美辛时，预期排卵时间的PGE和PGF水平（即2小时后）总是等于或低于未被hCG刺激的卵泡中的PG水平，但排卵率为46.7 +/-12.3%，30 +/-7.7%、14.3 +/-9.3%和0%。因此，研究结果对PGs在排卵过程中的具体作用提出了质疑。
This study determined specifically when ovarian prostaglandins (PGs) increase during ovulation and how effectively different doses of indomethacin inhibit PGs and ovulation. Rabbit ovarian follicles were removed at hourly intervals after stimulating the animals with hCG (50 IU/kg). The follicles were homogenized in 0.1 M acetate buffer (pH 4.5), and the PGE and PGF in the extracts were measured by RIA. Ovulation rates were determined by calculating the percentage of mature follicles that ruptured after stimulation by hCG. Before hCG, the normal levels of PGE and PGF were 111.1 +/- 14.7 and 51.0 +/- 6.6 pg/mg follicle, respectively. By 2 h after hCG treatment, PGE and PGF both increased to 162.6 +/- 17.0 and 80.6 +/- 13.3 pg/mg follicle, respectively. Approximately 5 h later, there was a second, sharper increase in both PGs which peaked at 652.6 +/- 63.5 and 345.4 +/- 32.3 pg/mg follicle, respectively, 10 h after hCG treatment, i.e. at the expected time of ovulation. We found that regardless of whether indomethacin was given early or late during the ovulation process, this agent significantly reduced follicular PG production within 5 min after its administration. For example, only 5 min after 10 mg/kg indomethacin were administered 8 h after hCG, PGE and PGF dropped from 317.7 +/- 50.0 and 125.0 +/- 10.5 pg/mg follicle to 93.3 +/- 17.4 and 49.3 +/- 10.5 pg/mg follicle, respectively. Unexpectedly, when graded doses of indomethacin were administered either 1 or 8 h after hCG, there was not a statistically significant correlation between follicular PG levels and ovulation rate. For example, when doses of 1.25, 2.5, 5.0, and 10.0 mg/kg indomethacin were given 8 h after hCG, the PGE and PGF levels at the expected time of ovulation (i.e. 2 h later) were always equal to or less than the PG levels in follicles that had not been stimulated by hCG, yet ovulation proceeded at rates of 46.7 +/- 12.3%, 30 +/- 7.7%, 14.3 +/- 9.3%, and 0%, respectively. Therefore, the results raise questions about the specific role of PGs in the ovulation process.