Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes
Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes
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骨髓间充质干细胞上调出生后大鼠心室肌细胞的瞬时外向钾电流
DOI：
10.1016/j.yjmcc.2009.03.002
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发表时间：
2009-07-01
影响因子：
5
通讯作者：
Yang Baofeng
中科院分区：
文献类型：
--
作者：
Cai Benzhi;Zhao Limei;Yang Baofeng
骨髓间充质干细胞（BMSC）移植已被证明可以有效地改善实验动物和心肌梗死和心脏肥大患者的心功能。骨髓间充质干细胞通过抑制心肌细胞凋亡、减轻心肌炎症等作用对心肌细胞发挥重要作用。本研究旨在探讨骨髓间充质干细胞对新生大鼠心室肌细胞电生理特性的影响。骨髓间充质干细胞和NRVM以1：10的比例与半透膜间接共培养。结果发现，与单一培养的NRVM相比，共培养的NRVM瞬时外向钾电流（I-to）明显增加，并伴随着I-to的激活、失活和恢复的显著变化，同时，共培养的NRVM中编码I-to通道的K（V）4.2mRNA的表达量明显高于单一培养的NRVM。BMSCs培养液中碱性成纤维细胞生长因子（bFGF）和胰岛素生长因子-1（IGF-1）水平升高。bFGF上调K（V）4.2mRNA表达，增强I-to电流，IGF-1无此作用。总而言之，我们得出结论，BMSC至少部分地通过分泌bFGF上调NRVMs的I-to，从而上调K（V）4.2表达并改变I-to的动力学。(C)2009 Elsevier Inc. All rights reserved.
Bone marrow mesenchymal stem cell (BMSC) transplantation has been shown to effectively improve cardiac function in experimental animals and patients with myocardial infarction and heart hypertrophy. BMSCs exert potent effects on cardiomyocytes through the inhibition of cardiac apoptosis, the attenuation of cardiac inflammation, etc. However, novel biological actions of BMSCs on cardiomyocytes remain to be explored. The present study was designed to investigate whether BMSCs affect electrophysiological features of neonatal rat ventricular myocytes (NRVMs). BMSCs and NRVMs were indirectly co-cultured at a ratio of 1:10 with a semipermeable membrane. We found that compared with mono-cultured NRVMs, co-cultured NRVMs exhibited an obvious increase of transient outward potassium current (I-to), accompanied by significant changes in activation, inactivation and recovery of It, Meanwhile, K(V)4.2 mRNA which encodes the channel carrying I-to was more abundant in co-cultured NRVMs than mono-cultured NRVMs. The increases in basic fibroblast growth factor (bFGF) and insulin growth factor-1 (IGF-1) levels were observed in culture medium of BMSCs. bFGF but not IGF-1 upregulated the K(V)4.2 mRNA expression and enhanced I-to currents. Taken together, we conclude that BMSCs upregulate I-to of NRVMs, at least partially, by secreting bFGF that in turn upregulates K(V)4.2 expression and alters the kinetics of I-to. (C) 2009 Elsevier Inc. All rights reserved.