Simultaneous electrochemical measurements of oxygen and dopamine in vivo.
Simultaneous electrochemical measurements of oxygen and dopamine in vivo.
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DOI：
10.1021/ac00001a005
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发表时间：
1991
影响因子：
7.4
通讯作者：
Zimmerman Jb;Wightman Rm
中科院分区：
文献类型：
--
作者：
Zimmerman Jb;Wightman Rm
快速扫描循环伏安法是一种经过验证的体内检测儿茶酚胺神经递质的分析方法，已扩展到同时测定分子氧 (O2)。在涂有全氟化离子交换材料的碳纤维圆盘电极上以 400 V/s 的扫描速率记录循环伏安图。在这些条件下，O2 的峰值电流出现在 -1.2 V 附近。在流动注射实验中，这些电极对多巴胺和 O2 的阶跃变化做出响应，半上升时间小于 200 毫秒。伏安峰值电流与流速无关，表明扩散限制响应不受对流影响。对体内基质中存在的几种化合物（腺苷、谷胱甘肽和 NAD 以及谷氨酸、乳酸和尿酸）进行了测试，结果表明不会干扰 O2 的伏安信号。这些电极在体内保持稳定的响应至少 6 小时。它们已被用来测量麻醉大鼠尾状核细胞外液中多巴胺和氧气的瞬时增加，以响应电刺激。
Fast-scan cyclic voltammetry, a demonstrated analytical method for the in vivo detection of catecholamine neurotransmitters, is extended to the simultaneous determination of molecular oxygen (O2). Cyclic voltammograms were recorded at a scan rate of 400 V/s at carbon-fiber disk electrodes coated with a perfluorinated ion-exchange material. The peak current for O2 occurs near -1.2 V under these conditions. In flow-injection experiments, these electrodes respond to step changes in dopamine and O2 with a half-rise time of less than 200 ms. The voltammetric peak current is independent of flow rate, indicating a diffusion-limited response unaffected by convection. Several compounds present in the in vivo matrix (adenosine, glutathione, and NAD and glutamic, lactic, and uric acids) were tested and shown not to interfere with the voltammetric signal for O2. These electrodes maintain a stable response in vivo for at least 6 h. They have been used to measure transient increases in both dopamine and O2 in the extracellular fluid of the caudate nucleus of an anesthetized rat in response to an electrical stimulus.