Optimization of fabrication parameters to produce chitosan-tripolyphosphate nanoparticles for delivery of tea catechins
Optimization of fabrication parameters to produce chitosan-tripolyphosphate nanoparticles for delivery of tea catechins
复制标题
DOI：
10.1021/jf801111c
复制
发表时间：
2008-08-27
影响因子：
6.1
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
--
作者：
Hu, Bing;Pan, Chenliang;Zeng, Xiaoxiong
这项工作研究了聚阴离子引发的凝胶化过程，用于制造壳聚糖三聚磷酸盐（CS-TPP）纳米颗粒，该纳米颗粒旨在用作传递茶儿茶素的载体。结果表明，CS-TPP纳米粒子的粒径和表面电荷可以通过制造条件控制。为了制备负载茶儿茶素的CS-TPP纳米粒，系统考察了CS与茶儿茶素的接触时间、CS分子量、CS浓度、CS-TPP质量比、CS溶液初始pH值和茶儿茶素浓度等调节条件对茶儿茶素包封率和体外释放曲线的影响。研究发现CS-TPP纳米颗粒中茶儿茶素的包封率在24%至53%之间。此外，FT-IR分析表明，茶儿茶素与CS之间在负载茶儿茶素的CS-TPP纳米颗粒的形成过程中发生了共价键和氢键。此外，对茶儿茶素体外释放曲线的研究表明，使用 CS-TPP 纳米颗粒可以实现茶儿茶素的控制释放。
This work investigated the polyanion-initiated gelation process in fabricating chitosan-tripolyphosphate (CS-TPP) nanoparticles intended to be used as carriers for delivering tea catechins. The results demonstrated that the particle size and surface charge of CS-TPP nanoparticles could be controlled by fabrication conditions. For preparation of CS-TPP nanoparticles loaded with tea catechins, the effects of modulating conditions including contact time between CS and tea catechins, CS molecular mass, CS concentration, CS-TPP mass ratio, initial pH value of CS solution, and concentration of tea catechins on encapsulation efficiency and the release profile of tea catechins in vitro were examined systematically. The study found that the encapsulation efficiency of tea catechins in CS-TPP nanoparticles ranged from 24 to 53%. In addition, FT-IR analysis showed that the covalent bonding and hydrogen bonding between tea catechins and CS occurred during the formation of CS-TPP nanoparticles loaded with tea catechins. Furthermore, studies on the release profile of tea catechins in vitro demonstrated that the controlled release of tea catechins using CS-TPP nanoparticles was achievable.