Oxysterol-binding protein-related protein 2 is not essential for Phytophthora sojae based on CRISPR/Cas9 deletions
Oxysterol-binding protein-related protein 2 is not essential for Phytophthora sojae based on CRISPR/Cas9 deletions
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基于 CRISPR/Cas9 缺失，氧甾醇结合蛋白相关蛋白 2 对于大豆疫霉并非必需
DOI：
10.1111/1758-2229.12638
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发表时间：
2018
影响因子：
3.3
通讯作者：
Tyler BM
中科院分区：
文献类型：
--
作者：
Miao Jianqiang;Lin Dong;Liu Xili;Li Xiuhuan;Tyler Brett M.;Tyler Brett M.;Liu XL;Tyler BM
Oxysterol‐binding protein (OSBP)‐related proteins (ORPs) are a large conserved family of lipid transfer proteins in eukaryotes. In oomycetes, some ORPs are the target of the novel oomycide oxathiapiprolin. By searching thePhytophthora sojaegenome database, two ORP proteins, PsORP1 (Protein ID: 558498) and PsORP2 (Protein ID: 470921), were found. Here, we investigated the biological function ofPsORP2. The expression level ofPsORP2was higher in germinated cysts and late infection than in other developmental stages. However, deletion ofPsORP2using CRISPR/Cas9 had no significant effect on sporangia production, zoospore production, cyst germination, oospore production, virulence or oxathiapiprolin sensitivity.PsORP1also was not upregulated in ΔPsORP2transformants. Collectively, our studies demonstrate that PsORP2 is not an essential protein for development or virulence inP. sojaeunder the conditions we tested.
