In vitro development of B lymphocytes from long-term cultured precursor cells.
In vitro development of B lymphocytes from long-term cultured precursor cells.
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来自长期培养的前体细胞的 B 淋巴细胞的体外发育。
DOI：
10.1073/pnas.83.2.441
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发表时间：
1986
影响因子：
11.1
通讯作者：
O. Witte
中科院分区：
文献类型：
--
作者：
K. Denis;O. Witte
建立了一个研究B淋巴细胞前体分化的体外系统。使用两组单独的培养条件来控制B淋巴细胞前体生长与分化。第一种是模仿Dexter及其同事的骨髓培养系统[Dexter，T. M. & Testa，N. G.（1976）在Methods in Cell Biology，ed. Prescott，D. M.（Academic，纽约），第14卷，pp. 387-395]，允许前体复制，而第二组培养条件允许分化为B淋巴细胞谱系的更成熟成员。从一组条件到另一组条件的转变使我们能够在5周的时间内跟踪从前体细胞到前B和B淋巴细胞的这种分化的动力学。由此产生的人口B系细胞合成异质性免疫球蛋白分子，通过二维凝胶电泳分析，并具有异质性阵列的免疫球蛋白基因重排，表明起源于非常早期的未定型的前体。这种体外系统将允许研究调节B淋巴细胞分化的基因，并将有助于分离B细胞发育途径中的细胞中间体。
An in vitro system for the study of B-lymphocyte differentiation from precursor elements has been established. Two separate sets of culture conditions are utilized to control B-lymphocyte precursor growth versus differentiation. The first, modeled after the bone marrow culture system of Dexter and colleagues [Dexter, T. M. & Testa, N. G. (1976) in Methods in Cell Biology, ed. Prescott, D. M. (Academic, New York), Vol. 14, pp. 387-395], allows precursor replication whereas the second set of culture conditions is permissive for differentiation to more mature members of the B-lymphocyte lineage. The shift from one set of conditions to the other allows us to follow the kinetics of this differentiation from precursor cells to pre-B and B lymphocytes over a 5-week period. The resulting population of B-lineage cells synthesizes heterogeneous immunoglobulin molecules as analyzed by two-dimensional gel electrophoresis and has a heterogeneous array of immunoglobulin gene rearrangements, suggesting an origin from very early uncommitted precursors. This in vitro system will allow the study of genes that regulate B-lymphocyte differentiation and will aid in the isolation of the cellular intermediates in the B-cell developmental pathway.