Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections.
Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections.
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塑料和石蜡包埋的组织切片中酶活性的组织化学演示。
DOI：
10.3109/10520297909110667
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
B. Schofield
中科院分区：
文献类型：
--
作者：
S. Higuchi;M. Suga;A. Dannenberg;B. Schofield
酶的组织化学染色通常在冰冻切片上进行。该报告列出了在塑料包埋和常规石蜡包埋组织中证明酯酶、酸性磷酸酶、β-半乳糖苷酶和细胞色素氧化酶所需的较长孵育时间。包埋在塑料中的切片，即水溶性甲基丙烯酸酯（Polyscience 的 JB-4）并切成 2 微米，在组织细节和组织化学反应产物的定位方面都远远优于冷冻切片和石蜡包埋切片。
Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, beta-galactosidase, and cytochrome oxidase in plastic embedded and ruotine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 micrometers, were far superior to frozen sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.