[Studies on influence of Siwu decoction and its composite drugs on chemical-induced blood-deficiency model mice].
[Studies on influence of Siwu decoction and its composite drugs on chemical-induced blood-deficiency model mice].
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DOI：
10.4268/cjcmm20111820
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发表时间：
2011-09
期刊：
影响因子：
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通讯作者：
Min Zhu;J. Duan;Yuping Tang;S. Su;Yong-qing Hua;Jianming Guo;D. Qian
中科院分区：
文献类型：
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作者：
Min Zhu;J. Duan;Yuping Tang;S. Su;Yong-qing Hua;Jianming Guo;D. Qian
目的探讨四物汤及其复方药物对乙酰苯肼、环磷酰胺所致血虚模型小鼠的影响。方法采用乙酰苯肼和环磷酰胺复制血虚模型小鼠。采用全自动血液学分析仪检测外周血图。采用加权法测定肝脏指数和脾脏指数；采用atp酶检测试剂盒检测红细胞膜Na+ - K+ - atp酶/Ca2+ - Mg2+ - atp酶活性。流式细胞术检测骨髓细胞的细胞周期。结果当归、白芍对外周血血流图的影响最大。白芍、当归对药组和白芍组对肝脏指数的影响最大。各药物、药对、药组及方剂均对脾指数有影响。对红细胞膜Na+ - K+ - atp酶活性，生地黄、当归等药物对和药物组表现出最有效的活性。所有药物、药物对、药物组及配方均对受损骨髓细胞有保护作用。结论四物汤及其复方药物对血虚模型小鼠均有影响，但作用强度不同。当归和白芍对血虚模型小鼠的保护作用最明显。
OBJECTIVE To investigate Siwu decoction and its composite drugs on the blood-deficiency model mice induced by acetylphenyhydrazine and cyclophosphamide. METHOD Acetylphenyhydrazine and cyclophosphamide were used to copy the blood-deficiency model mice. Automatic hematology analyzer was used to test the peripheral hemogram. Weighting method was used to test the liver index and spleen index; Kits for ATPase test was used to test the activities of Na+ - K+ - ATPase/Ca2+ - Mg2+ - ATPase in erythrocyte membrane. Flow cytometry was used to test the bone marrow cells' cell cycle. RESULT Angelicar Sinensis Radix and Paeoniae Radix Alba had the most effective activity on the peripheral hemogram. Paeoniae Radix Alba, the drug pair including Angelicar Sinensis Radix and the drug- group including Paeoniae Radix Alba had the most effective activity on the liver index. All the drugs, drug-pairs, drug-groups and the formula had effect on the spleen index. To the activity of Na+ - K+ - ATPase in erythrocyte membrane, Rehmanniae Radix Praeparata, the drug-pairs and drug-groups including Angelicar Sinensis Radix exhibited the most effective activity. All the drugs, drug-pairs, drug-groups and the formula had the protective effect on the damaged bone marrow cells. CONCLUSION Siwu decoction and its composite drugs all had effect on the blood-deficiency model mice, but the action intensity was different. Angelicar Sinensis Radix and Paeoniae Radix Alba exhibited the most effective activity to the protection of the blood-deficiency model mice.