Co-electrospun poly(lactide-co-glycolide), gelatin, and elastin blends for tissue engineering scaffolds
Co-electrospun poly(lactide-co-glycolide), gelatin, and elastin blends for tissue engineering scaffolds
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DOI：
10.1002/jbm.a.30833
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发表时间：
2006-12-15
影响因子：
4.9
通讯作者：
Lelkes, Peter I.
中科院分区：
文献类型：
--
作者：
Li, Mengyan;Mondrinos, Mark J.;Lelkes, Peter I.
在这项研究中，我们描述了由合成和天然材料组成的复合支架，其理化特性适合组织工程应用。纤维支架由合成生物可降解聚合物（聚乳酸-乙醇酸共聚物，PLGA，10% 溶液）和两种天然蛋白质明胶（变性胶原蛋白，8% 溶液）和（x-弹性蛋白（20% 溶液））以 3:1:2 和 2:2:2 (v/v/v) 的比例共混纺丝而成。所得的 PLGA-明胶-弹性蛋白 (PGE) 纤维是均匀的外观上，平均直径为 380 ± 80 mn，比在相同条件下由起始材料（PLGA，780 ± 200 nm；明胶，447 ± 1.23 nm；弹性蛋白，1060 ± 170 nm）制成的纤维小得多。水合后，PGE 纤维膨胀至平均纤维直径为 963 ± 132 nm，但没有分解。重要的是，PGE 支架在水环境中稳定，无需交联，并且比纯弹性蛋白纤维制成的支架更有弹性。为了研究 PGE 的细胞相容性，我们在纤维 PGE 支架上培养 H9c2 大鼠心肌成肌细胞和大鼠骨髓基质细胞 (BMSC)，我们发现成肌细胞在支架上的生长与在组织培养塑料上的生长相同或稍好。 PGE 构建体的组织学特征表明，BMSC 渗入支架中心并在接种后不久开始增殖。我们的结果表明，由 PGE 和类似的天然和合成聚合物的仿生混合物制成的纤维支架可用于工程化软组织，例如心脏、肺和血管 (c) 2006 Wiley periodicals, Inc.。
In this study, we describe composite scaffolds composed of synthetic and natural materials with physicochemical properties suitable for tissue engineering applications. Fibrous scaffolds were co-electrospun from a blend of a synthetic biodegradable polymer (poly(lactic-co-glycolic acid), PLGA, 10% solution) and two natural proteins, gelatin (denatured collagen, 8% solution) and (x-elastin (20% solution) at ratios of 3:1:2 and 2:2:2 (v/v/v). The resulting PLGA-gelatin-elastin (PGE) fibers were homogeneous in appearance with an average diameter of 380 80 mn, which was considerably smaller than fibers made under identical conditions from the starting materials (PLGA, 780 +/- 200 nm; gelatin, 447 +/- 1.23 nm; elastin, 1060 170 nm). Upon hydration, PGE fibers swelled to an average fiber diameter of 963 +/- 132 nm, but did not disintegrate. Importantly, PGE scaffolds were stable in an aqueous environment without crosslinking, and were more elastic than those made of pure elastin fibers. To investigate the cytocompatibility of PGE, we cultured H9c2 rat cardiac myoblasts and rat bone marrow stromal cells (BMSCs) on fibrous PGE scaffolds. We found that myoblasts grew equally as well or slightly better on the scaffolds than on tissue-culture plastic. Microscopic evaluation confirmed that myoblasts reached confluence on the scaffold surfaces while simultaneously growing into the scaffolds. Histological characterization of the PGE constructs indicated that BMSCs penetrated into the center of scaffolds and began proliferating shortly after seeding. Our results suggest that fibrous scaffolds made of PGE and similar biornimetic blends of natural and synthetic polymers may be useful for engineering soft tissues, such as heart, lung, and blood vessels. (c) 2006 Wiley Periodicals, Inc.