THE DETAILED DISTRIBUTION OF HLA-A-ANTIGEN, B-ANTIGEN, C-ANTIGEN IN NORMAL HUMAN ORGANS
THE DETAILED DISTRIBUTION OF HLA-A-ANTIGEN, B-ANTIGEN, C-ANTIGEN IN NORMAL HUMAN ORGANS
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DOI：
10.1097/00007890-198409000-00018
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发表时间：
1984-01-01
期刊：
影响因子：
6.2
通讯作者：
MORRIS, PJ
中科院分区：
文献类型：
--
作者：
DAAR, AS;FUGGLE, SV;MORRIS, PJ
采用靶向HLA-ABC抗原重链的单克隆抗体PA2.6，研究MHC I类抗原的详细组织分布。从器官捐献者或手术标本中获得新鲜的人体正常组织，并在移除后1-2小时内在液氮中快速冷冻。然后使用敏感的过氧化物酶-抗过氧化物酶免疫组织学技术研究冷冻切片。I类抗原可以在大多数（但不是全部）体内有核细胞上检测到。在一些组织中，包括甲状腺、甲状旁腺、垂体和胰岛的内分泌细胞，以及胃粘膜、心肌、骨骼肌和一些标本的肝细胞中，它们只能微弱地检测到。精子在睾丸中呈阳性染色，但当它们向上移动到附睾时，I类抗原不再被检测到。ⅰ类抗原未检出。I类抗原在角膜内皮、十二指肠部分布伦纳氏腺、绒毛滋养细胞、中枢神经系统神经元、胰腺外分泌部分和腮腺腺泡细胞中未检出。I类抗原显然不像以前认为的那样普遍存在。
A monoclonal antibody, PA2.6, directed against the heavy chain of HLA-ABC antigens was used to study the detailed tissue distribution of MHC class I antigens. Normal tissues from throughout the human body were obtained fresh from organ donors or operative specimens and were snap-frozen in liquid nitrogen within 1-2 h of removal. Frozen sections were then studied using a sensitive peroxidase-antiperoxidase immunohistological technique. Class I antigens could be detected on most, but not all, the nucleated cells in the body. They were only weakly detectable in several tissues including endocrine cells in the thyroid, parathyroid, pituitary and islets of Langerhans in the pancreas and on gastric mucosa, the myocardium, skeletal muscle and hepatocytes in some of the specimens. Spermatozoa were positively stained in the testis, but as they moved up into the epididymis class I antigens were no longer detectable. Class I antigens were not detectable. Class I antigens were not detectable on corneal endothelium, some Brunner''s glands in the duodenum, villous trophoblast, CNS neurons, the exocrine portion of the pancreas, and acinar cells in the parotid. Class I antigens apparently are not ubiquitous, as previously thought.