Human papillomavirus detection by penile site in young men from Kenya
Human papillomavirus detection by penile site in young men from Kenya
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DOI：
10.1097/olq.0b013e318065b8ef
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发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Bailey, Robert C.
中科院分区：
文献类型：
--
作者：
Smith, Jennifer S.;Moses, Stephen;Bailey, Robert C.
背景资料：有限的数据是可用的，是否从阴茎轴或尿道采样增加检测阴茎HPV感染的男性超出了发现在龟头和冠状sulcus.Methods：在一项随机临床试验中，阴茎采样的验证研究进行了基苏穆，肯尼亚。邀请年轻男性（18-24岁）使用预润湿的Dacron拭子提供阴茎脱落细胞，以确定HPV检测的最佳部位。结果：98例HIV阴性、未行包皮环切术的男性青年患者，均行β-珠蛋白基因PCR和HPV DNA GP 5 +/6+ PCR检测。阴茎HPV感染率因解剖部位而异：阴茎头、冠状沟和内包皮组织脱落细胞中为50%;阴茎干和外包皮组织中为43%;尿道中为18%（P < 0.0001）。对于每个解剖部位，超过87%的样本是β-珠蛋白阳性。除了在龟头/冠状沟中发现的之外，尿道取样导致HPV阳性率没有增加，而干取样导致总体HPV阳性率增加7.3%。高危型HPV阳性率因解剖部位而异：龟头/冠状沟为39%，干部为31%，尿道为13%（P < 0.0001）。HPV 16型是最常见的类型identified.Discussion：阴茎HPV的患病率约为50%的年轻男子在基苏穆，肯尼亚。在男性中进行HPV检测的尿道取样与从龟头/冠状沟和阴茎干取样所发现的相比，没有增加HPV检测的灵敏度。这些数据将有助于为HPV传播动力学研究以及HPV预防性疫苗对男性阴茎HPV携带的有效性提供信息。
Background: Limited data are available on whether sampling from the penile shaft or urethra increases detection of penile HPV infection in men beyond that found in the glans and coronal sulcus.Methods: Within a randomized clinical trial, a validation study of penile sampling was conducted in Kisumu, Kenya. Young men (18-24 years) were invited to provide penile exfoliated cells using prewetted Dacron swabs to determine the best site for HPV detection. beta-Globin gene PCR and HPV DNA type GP5+/6+ PCR status were ascertained from 3 anatomical sites.Results: A total of 98 young HIV-seronegative, uncircumcised men participated. Penile HPV prevalence varied by anatomical site: 50% in penile exfoliated cells from the glans, coronal sulcus, and inner foreskin tissue; 43% in the shaft and external foreskin tissue; and 18% in the urethra (P < 0.0001). For each anatomical site, over 87% of samples were beta-globin positive. Beyond that found in the glans/coronal sulcus, urethral sampling resulted in no increase in HPV positivity and shaft sampling resulted in an additional 7.3% of overall HPV positivity. The prevalence of high-risk HPV positivity varied by anatomical site: 39% in glans/coronal sulcus, 31% in shaft, and 13% in the urethra (P < 0.0001). HPV 16 was the most common type identified.Discussion: Penile HPV prevalence was approximately 50% among young men in Kisumu, Kenya. Urethral sampling for HPV detection in men added no sensitivity for HPV detection over that found from sampling the glans/coronal sulcus and penile shaft. These data will help inform studies on HPV transmission dynamics, and on the efficacy of HPV prophylactic vaccines on penile HPV carriage in men.