FUSOME ASYMMETRY AND OOCYTE DETERMINATION IN DROSOPHILA
FUSOME ASYMMETRY AND OOCYTE DETERMINATION IN DROSOPHILA
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DOI：
10.1002/dvg.1020160104
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
SPRADLING, AC
中科院分区：
文献类型：
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作者：
LIN, HF;SPRADLING, AC
在果蝇卵子发生的早期阶段，由称为成囊细胞的祖细胞产生含有16个相互连接的细胞（囊细胞）的生殖系囊，所述祖细胞经历四轮同步的不完全分裂。在囊肿形成过程中，一个专门的，富含血影蛋白的细胞质区域称为融合体，穿过细胞间的连接（环管），连接单个囊细胞。随后，15个囊细胞开始将特定的RNA和其他成分转运到剩余的细胞，即未来的卵母细胞中。我们使用fu some特异性抗体来表征囊肿形成的早期阶段。在第一次成囊细胞分裂过程中，一团球形的融合体物质（“光谱体”）只与有丝分裂纺锤体的一极相连，表明这种分裂是不对称的。在随后的三次分裂中，生长的融合体总是与保留在母细胞中的每个有丝分裂纺锤体的极相关联，并且仅在每次分裂完成后延伸穿过新形成的环道。这些观察结果表明，融合体有助于建立一个系统的定向运输囊细胞之间的卵母细胞决定的基础。(C)1995 Wiley-Liss，Inc.
Germline cysts containing 16 interconnected cells (cystocytes) are produced at an early stage of Drosophila oogenesis by progenitor cells known as cystoblasts that undergo four synchronous rounds of incomplete division. During cyst formation, a region of specialized, spectrin-rich cytoplasm called the fusome traverses the intercellular connections (ring canals), linking individual cystocytes. Subsequently, 15 cystocytes begin to transport specific RNAs and other components into the remaining cell, the future oocyte. We used fu some-specific antibodies to characterize the early stages of cyst formation. During the first cystoblast division, a spherical mass of fusome material (the ''spectrosome'') was associated with only one pole of the mitotic spindle, revealing that this division is asymmetric. During the subsequent three divisions, the growing fusome always associated with the pole of each mitotic spindle that remained in the mother cell, and only extended through the newly formed ring canals after each division was completed. These observations suggest that fusomes help establish a system of directional transport between cystocytes that underlies oocyte determination. (C) 1995 Wiley-Liss, Inc.