MICROSCOPY without lenses
MICROSCOPY without lenses
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DOI：
10.1063/pt.3.3693
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发表时间：
2017-09-01
期刊：
影响因子：
3.5
通讯作者：
Ozcan, Aydogan
中科院分区：
文献类型：
--
作者：
McLeod, Euan;Ozcan, Aydogan
自发明以来，经典光学显微镜及其后续变体通常依赖于一个关键元件：物镜透镜。物镜透镜可以是单个或复合透镜，其通常具有短焦距和大数值孔径NA = nsin θ，其中n是物镜透镜和样品之间的介质的折射率，θ是透镜的最大接受角。短焦距便于将物镜并入具有大放大率的光学系统中，使得微观物体可以通过人眼或数码相机观察。物镜的大数值孔径使得设计良好的成像系统能够分辨微观特征，其对于非相干光的经典极限为约λ/2NA，其中λ是光的波长。
Since its invention, the classical optical microscope and its successive variants have generally relied on one key element: the objective lens. The objective lens can be a single or compound lens that typically has a short focal length and large numerical aperture, NA = n sin theta, where n is the refractive index of the medium between the objective lens and the sample, and theta is the maximum acceptance angle of the lens. The short focal length facilitates the incorporation of the objective into an optical system with a large magnification so that microscopic objects can be observed by human eye or with a digital camera. The large numerical aperture of the objective enables a well-designed imaging system to resolve microscopic features down to the classical limit of approximately lambda/2NA for incoherent light, where lambda is the wavelength of the light.