Different organization of intermediate filaments in columnar cells of rat large intestinal mucosa as revealed by confocal laser scanning microscopy and quick-freezing and deep-etching method
Different organization of intermediate filaments in columnar cells of rat large intestinal mucosa as revealed by confocal laser scanning microscopy and quick-freezing and deep-etching method
复制标题
共聚焦激光扫描显微镜和速冻深蚀法揭示大鼠大肠粘膜柱状细胞中间丝的不同组织
作者：
A. Hemmi;A. Komiyama;A. Kawaoi;R. Katoh,;K. Suzuki;S. Ohno;Y. Fujii
应用激光共聚焦扫描显微镜和速冻深蚀法研究了大鼠大肠粘膜柱状细胞分化与中间丝超微结构变化的关系。未成熟柱状细胞中IF的一个特征是具有突出分支的微束形成，其组织了网状结构。微束，这似乎是由两个或两个以上的IF在侧到侧的方式连接形成的，松散地分布在整个细胞质。相反，在成熟的柱状细胞中，IF密集分布在细胞质中的终网下方和侧膜区域的上部下方，而细胞质的其他区域仅含有少量的IF。未观察到微束形成，很少发现分支。IF在成熟柱状细胞的特定区域表达，其分布和密度的变化显然代表了细胞内分化的特征。这表明，在未成熟的柱状细胞中经常观察到的微束IF的解离是这种类型的细胞获得功能极性的重要步骤。
The relationship between cell differentiation and ultrastructural changes of intermediate filaments (IF) was studied in columnar cells of large intestinal mucosa of rats by confocal laser scanning microscopy and quick-freezing and deep-etching method. A feature of the IF in immature columnar cells was minibundle formation with prominent branching, which organized the meshwork structures. The minibundles, which appeared to be formed by the attachment of two or more IF in side-to-side fashion, were loosely distributed throughout the cytoplasm. In contrast, in mature columnar cells, the IF were densely distributed under the terminal web in the cytoplasm and beneath the upper part of the lateral membrane regions, whereas the other areas of the cytoplasm contained only a small number of IF. Minibundle formation was not observed, and the branching was rarely identified. The changes in the distribution and density of IF, which are expressed in specific areas of mature columnar cells, apparently represent a characteristic of intracellular differentiation. It is suggested that the dissociation of minibundled IF, which was often observed in the immature columnar cells, is an important step in the acquisition of functional polarity in cells of this type.
DOI：
--
发表时间：
1985-03
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
D. Cooper;A. Schermer;T. Sun
通讯作者：
D. Cooper;A. Schermer;T. Sun