Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24/CCN2) during distraction osteogenesis
Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24/CCN2) during distraction osteogenesis
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DOI：
10.1007/s00774-004-0486-2
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Takigawa, M
中科院分区：
文献类型：
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作者：
Kadota, H;Nakanishi, T;Takigawa, M
为了研究结缔组织生长因子/肥大软骨细胞特异性基因产物24/CCN家族成员2(CTGF/Hcs24/CCN2)在大鼠股骨牵张成骨过程中的定位和表达，我们研究了11周龄雄性大鼠股骨牵张成骨过程中CTGF/Hcs24/CCN2的表达。我们在右侧股骨中段进行了截骨术。7d后(延迟期)，使用小型外固定器以0.25 mm/12h的速度开始牵张21d(牵张期)，然后进行7d的固位期。应用免疫组织化学染色、原位杂交和逆转录聚合酶链式反应(RT-PCR)检测CTGF/Hcs24在股骨牵张成骨过程中的定位和表达。免疫组织化学染色显示CTGF/Hcs24定位于截骨区周围骨形成区的各种细胞。在牵张期，CTGF/Hcs24mRNA不仅在肥大的软骨细胞和成骨细胞中表达，而且在软骨末端成骨部位的成纤维样细胞和间充质细胞中表达，在膜内成骨部位不仅在成骨细胞中表达，而且在成骨细胞和成纤维细胞样细胞中也有表达。RT-PCR显示牵张组CTGF/Hcs24基因表达水平高于非牵张组。这些结果揭示了CTGF/Hcs24在牵张成骨过程中的表达升高模式，提示CTGF/Hcs24可能在牵张成骨过程中的软骨内和膜内成骨过程中起一定作用。
To investigate the localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24/CCN family member 2 (CTGF/Hcs24/CCN2) during distraction osteogenesis in the rat femur, we studied a total of 54 male rats (11 weeks old). We performed osteotomy in the midshaft of the right femur. After 7 days (lag phase), distraction was started, at the rate of 0.25 mm/12 h for 21 days (distraction phase) by using a small external fixator, and this was followed by a 7-day consolidation phase. Localization and expression of CTGF/Hcs24 during distraction osteogenesis in the femur were examined by immunostaining, in situ hybridization, and reverse transcriptase polymerase chain reaction (RT-PCR). Immunostaining showed the localization of CTGF/Hcs24 in various cells located in the bone-forming area around the osteotomy site. During the distraction phase, in situ hybridization showed that CTGF/Hcs24 mRNA was expressed not only in hypertrophic chondrocytes and osteoblasts but also in fibroblast-like cells and mesenchymal cells at sites of end-ochondral ossification, and not only in osteoblasts but also in pre-osteoblasts and fibroblast-like cells at sites of intramembranous ossification. RT-PCR showed higher level expression of CTGF/Hcs24 mRNA in the distracted group than in the nondistracted group. These results revealed an elevated pattern of CTGF/Hcs24 mRNA expression during distraction osteogenesis, and suggest that CTGF/Hcs24 may play some roles in the endochondral and intramembranous ossification processes that occur during distraction osteogenesis.