ESTIMATION OF DISRUPTION OF ANIMAL-CELLS BY LAMINAR SHEAR-STRESS
ESTIMATION OF DISRUPTION OF ANIMAL-CELLS BY LAMINAR SHEAR-STRESS
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DOI：
10.1002/bit.260400903
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发表时间：
1992-11-01
影响因子：
3.8
通讯作者：
THOMAS, CR
中科院分区：
文献类型：
--
作者：
BORN, C;ZHANG, Z;THOMAS, CR
利用显微操作测得的细胞力学特性和层流场中细胞变形的模型，提出了一种预测层流剪切应力对动物细胞破坏的方法。预测的模型进行了比较，测量损失的细胞数量和生存能力的TB/C3小鼠杂交瘤剪切锥板粘度计剪切速率高达3950 s-1，剪切应力高达600 Nm-2，实现了通过增强粘度与葡聚糖。在所有情况下，实验结果和预测都在30%以内。这种良好的协议表明，它可能是可以使用动物细胞的机械性能的显微操作测量，以预测在更复杂的流场，如在生物反应器中的细胞损伤。
Using mechanical cell properties measured by micromanipulation, and a model of cell distortion in laminar flow fields, a method has been developed for predicting disruption of animal cells by laminar shear stresses. Predictions of the model were compared with measured losses of cell number and viability of TB/C3 murine hybridomas sheared in a cone and plate viscometer at shear rates up to 3950 s-1, and shear stresses up to 600 Nm-2, achieved by enhancement of viscosity with dextran. In all cases, the experimental results and predictions were within 30%. Such excellent agreement suggests it might be possible to use micromanipulation measurements of animal cell mechanical properties to predict cell damage in more complex flow fields, such as those in bioreactors.