Detection of human papillomavirus type 16 DNA in peritoneal washings from patients with cervical carcinoma.
Detection of human papillomavirus type 16 DNA in peritoneal washings from patients with cervical carcinoma.
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宫颈癌患者腹腔冲洗液中人乳头瘤病毒 16 型 DNA 的检测。
DOI：
10.1093/infdis/155.6.1349
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Burk,RD
中科院分区：
文献类型：
--
作者：
Anderson,LL;Ritter,DB;Kadish,AS;Runowicz,CD;Burk,RD
同事们 - 诊断人乳头瘤病毒 (HPV) 感染最敏感的方法是使用克隆的、特定类型的 HPV 探针进行 DNA 杂交 [1, 2]。通过使用此类技术，已在“-'800/0-90070”的癌前和恶性宫颈病变中检测到 HPV DNA [1-3]。此外，特定类型的 HPV，特别是 16 型和 18 型，最常与严重发育异常和恶性宫颈病变相关[1]。此外，在含有转移性宫颈癌的淋巴结中检测到了 HPV DNA；所鉴定的 HPV 类型与原发病变中发现的相同。 [4] 考虑到分子杂交检测 HPV DNA 的敏感性，我们推断应用这种方法可能提供一种客观的方法来评估从宫颈癌女性腹膜冲洗液中获得的细胞，本报告描述了从接受宫颈癌手术的三名患者中的两名患者的腹膜冲洗液中分离出的细胞中的 HPV DNA。三名患者因浸润性宫颈癌的根治性手术而入院。尖锐湿疣或在体检时有疾病的临床证据，在剖腹手术前，通过滴注和抽吸 10ml 0.9010 NaG 获得宫颈阴道冲洗液，如前所述 [5] 进入腹腔后，立即通过从腹膜腔和盆腔滴注和抽吸 100ml 0.9% NaG 获得腹膜冲洗液。死胡同。对大约 30 ml 的液体进行 HPV DNA 分析，其余的则提交进行常规细胞学评估。子宫切除后，从恶性宫颈组织中取出活检标本以确定是否存在 HPV。使用 HPV 6、11、16 和 18 型特异性 DNA 探针，通过限制性酶消化和分子杂交对从腹膜冲洗液细胞、组织活检标本和宫颈阴道细胞中分离的 DNA 进行分析 [5]。
COLLEAGUES-The most-sensitive method of diagnosing human papillomavirus (HPV) infection is by DNA hybridization using cloned, type-specific HPV probes [1, 2]. With the use of such techniques, HPV DNA has been detected in"-'800/0-90070 of premalignant and malignant cervical lesions [1-3]. Moreover, specific types of HPV, notably types 16and 18, have been most frequently associated with severely dysplastic and malignant cervical lesions [1]. In addition, HPV DNA has been detected in lymph nodes containing metastatic cervical cancer; the type of HPV identified was identical to that found in the primary lesion [4]. Given the sensitivity of molecular hybridization to detect HPV DNA, we reasoned that applying this method might provide an objective means of evaluating cells obtained from the peritoneal washings of women with cervical carcinoma. This report describes the identification of HPV DNA in cells isolated from the peritoneal washings from two of three patients undergoing surgery for cervical carcinoma.Three patients were admitted to the gynecologic oncology service for definitive surgical treatment of invasive cervical carcinoma. None of the three patients gave a history of condylomata accuminata or had clinical evidence of the disease upon physical examination. Before laparotomy, cervicovaginal washings were obtained by instilling and aspirating 10ml of 0.9010 NaG, as previously described [5]. Immediately upon entering the abdominal cavity, a peritoneal washing was obtained by instilling and aspirating"-'100 ml of 0.9% NaG from the peritoneal cavity and the pelvic cul-de-sac. Approximately 30 ml of this fluid was analyzed for HPV DNA, and the remainder was submitted for routine cytological evaluation. After the uterus was removed, biopsy specimens were taken from the malignant-appearing cervical tissue to determine the presence of HPV. DNA isolated from the cells of the peritoneal washings, tissue biopsy specimens, and cervicovaginal cells were analyzed by restriction-enzyme digestion and molecular hybridization using DNA probes specific for HPV types 6, 11, 16, and 18 [5].