Pyruvate kinase is post-translationally regulated by sirtuin 2 in Aedes aegypti mosquitoes.
Pyruvate kinase is post-translationally regulated by sirtuin 2 in Aedes aegypti mosquitoes.
复制标题
DOI：
10.1016/j.ibmb.2023.104015
复制
发表时间：
2023-11
影响因子：
3.8
通讯作者：
Scaraffia, Patricia Y.
中科院分区：
文献类型：
--
作者：
Petchampai, Natthida;Isoe, Jun;Balaraman, Prashanth;Oscherwitz, Max;Carter, Brendan H.;Sanchez, Cecilia G.;Scaraffia, Patricia Y.
关键词：
我们之前证明了埃及伊蚊丙酮酸激酶（AaPK）在蚊子碳和氮代谢的调节中起关键作用。为了进一步阐明AaPK是否可受Ae的翻译后调控。埃及伊蚊sirtuin 2 （AaSirt2）是一种依赖于NAD+的去乙酰化酶，可催化乙酰化赖氨酸残基的乙酰基去除，我们在非饥饿和饥饿的雌性蚊子中进行了一系列分析。通过qPCR和western blots分析AaSirt2的转录和蛋白谱，表明AaSirt2在第一个淋养循环中不同时间解剖的蚊子组织中对糖或血液摄食的反应有差异调节。我们还发现AaSirt2定位于脂肪体和胸腔的细胞质和线粒体细胞区室。western blotting在两个细胞区室中检测到多种赖氨酸乙酰化蛋白。此外，免疫沉淀蛋白的western blotting证明，在非饥饿和饥饿雌性的脂肪体和胸部的细胞质部分中，AaPK被赖氨酸乙酰化并与AaSirt2结合。与这些结果相关，我们还发现饥饿雌性脂肪体中rnai介导的AaSirt2的敲低显著降低了AaPK蛋白的丰度。值得注意的是，在四种不同的营养方案下，aasirt2缺陷雌性的存活率没有受到显著影响。综上所述，我们的数据显示AaPK受AaSirt2的翻译后调控。
We previously demonstrated that Aedes aegypti pyruvate kinase (AaPK) plays a key role in the regulation of both carbon and nitrogen metabolism in mosquitoes. To further elucidate whether AaPK can be post-translationally regulated by Ae. aegypti sirtuin 2 (AaSirt2), an NAD+-dependent deacetylase that catalyzes the removal of acetyl groups from acetylated lysine residues, we conducted a series of analysis in non-starved and starved female mosquitoes. Transcriptional and protein profiles of AaSirt2, analyzed by qPCR and western blots, indicated that the AaSirt2 is differentially modulated in response to sugar or blood feeding in mosquito tissues dissected at different times during the first gonotrophic cycle. We also found that AaSirt2 is localized in both cytosolic and mitochondrial cellular compartments of fat body and thorax. Multiple lysine-acetylated proteins were detected by western blotting in both cellular compartments. Furthermore, western blotting of immunoprecipitated proteins provided evidence that AaPK is lysine-acetylated and bound with AaSirt2 in the cytosolic fractions of fat body and thorax from non-starved and starved females. In correlation with these results, we also discovered that RNAi-mediated knockdown of AaSirt2 in the fat body of starved females significantly decreased AaPK protein abundance. Notably, survivorship of AaSirt2-deficient females maintained under four different nutritional regimens was not significantly affected. Taken together, our data reveal that AaPK is post-translationally regulated by AaSirt2.
登录
查看更多内容
影响因子：
4.6
作者：
通讯作者：
--
影响因子：
4.8
作者：
Liszt, G;Ford, E;Guarente, L
通讯作者：
Guarente, L
影响因子：
4.8
作者：
Hershberger, Kathleen A.;Abraham, Dennis M.;Hirschey, Matthew D.
通讯作者：
Hirschey, Matthew D.
影响因子：
3.4
作者：
Severo MS;Sakhon OS;Choy A;Stephens KD;Pedra JH
通讯作者：
Pedra JH
影响因子：
2.2
作者：
Scaraffia, Patricia Y.;Zhang, Qingfen;Thorson, Kelsey;Wysocki, Vicki H.;Miesfeld, Roger L.
通讯作者：
Miesfeld, Roger L.