Oriented Collagen-Based/Hydroxyapatite Matrices for Articular Cartilage Replacement
Oriented Collagen-Based/Hydroxyapatite Matrices for Articular Cartilage Replacement
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用于关节软骨置换的定向胶原/羟基磷灰石基质
DOI：
10.4028/www.scientific.net/kem.254-256.1083
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Schubert
中科院分区：
文献类型：
--
作者：
R. Zehbe;U. Gross;H. Schubert
关节软骨的机械刺激被认为是软骨形成的必要条件。在我们的研究中，我们使用来自股骨髁的动物软骨骨外植体，将其分为三个实验组（加载、卸载和对照组）。以dme为培养基，在无菌生理环境下进行上样。用组织学方法观察细胞形态和细胞活力。从体外力学研究中获得的知识现在被用于生产一种在生化成分、生物力学特性和带状组织结构上类似于天然软骨的结构化人工基质。然后将人工基质植入软骨细胞，并加载与我们发现的组织再生理想参数相同的参数。使用组织学和免疫组织学方法进行分析。
Mechanical stimulation of articular cartilage is known to be es sential for chondrogenesis. In our study we use animal cartilage-bone explants from the femur condylus, which were divided into three experimental groups (loaded, unloaded and controls). Loading was pe rformed in a sterile and physiological environment based on DME-medium. Cell morphology and cell vitality was observed by histological techniques. The knowledge gathered from the mecha nical in-vitro study is now used to produce a structured artificial matrix resembling nati ve cartilage in biochemical composition, biomechanical properties and zonal tissue structure. The art ificial matrix is then seeded with chondrocytes and loaded with the same parameters we found t o be ideal for tissue regeneration. Analysis is performed using histological and immuno-histological m ethods.