Radioresistant mutants of L5178Y cells.
Radioresistant mutants of L5178Y cells.
复制标题
L5178Y 细胞的放射抗性突变体。
DOI：
10.2307/3572720
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发表时间：
1969
影响因子：
3.4
通讯作者：
V. Courtenay
中科院分区：
文献类型：
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作者：
V. Courtenay
用氚化水掺入培养液中，以4.8rads/h的β射线连续照射L5178 Y白血病细胞，获得抗辐射培养物。暴露39天或更长时间（总剂量≥ 4.6 krad）后，个别培养物中辐射抗性的发展被证明是由于在辐射过程中产生抗性突变体。在来自突变体的抗性细胞株中，染色体计数与40的模态值相比没有变化，并且在DBA/2小鼠中生长的能力没有变化。在没有辐射的情况下，抗性培养物的生长速度比正常细胞慢得多。在连续照射下，突变体在生长速度和死亡细胞的比例方面彼此不同。耐药细胞株以4.8拉德/小时的速度维持了近一年，在此期间，耐药较低的培养物的生长速度以对应于进一步突变的发生的步骤加快，最终达到约100拉德/小时。
Radioresistant cultures of the L5178Y leukemia were obtained during continuous β-irradiation at 4.8 rads/hour by means of tritiated water incorporated in the culture medium. The development of radiation resistance in individual cultures after exposures of 39 days or more (total dose ≥ 4.6 krads) was shown to be due to the production of resistant mutants during the course of irradiation. In the resistant cell strains derived from the mutants the chromosome count was unchanged from the modal value of 40, and the ability to grow in the DBA/2 mouse was unchanged. In the absence of radiation, resistant cultures grew rather more slowly than normal cells. Under continuous irradiation the mutants differed from each other in growth rate and in the proportion of dead cells present. The resistant cell strains were maintained at 4.8 rads/hour for nearly a year, during which the growth rate of the less resistant cultures speeded up in steps corresponding to the occurrence of further mutations, eventually reaching abou...