Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures.
Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures.
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在富集的原代皮质星形胶质细胞培养物中诱导一氧化氮合酶 2 表达并测量一氧化氮的产生。
DOI：
10.1007/978-1-61779-452-0_17
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hewett,SandraJ
中科院分区：
文献类型：
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作者：
Hewett,JamesA;Hewett,SandraJ
Astrocytes produce numerous mediators under conditions of inflammation in the central nervous system. One such mediator is nitric oxide (NO) derived from nitric oxide synthase-2 (NOS-2), the high output, inducible NOS isoform. Expression of NOS-2 and production of NO can be stimulated in astrocyte cultures by combinations of cytokines and lipopolysaccharide, a gram-negative bacterial endotoxin. This chapter details methods to induce and analyze NOS-2 expression and NO production in astrocyte cultures.
