Mini-and microsatellite mutations in children from Chernobyl accident cleanup workers
Mini-and microsatellite mutations in children from Chernobyl accident cleanup workers
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DOI：
10.1016/j.mrgentox.2004.01.003
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发表时间：
2004-04-11
影响因子：
1.9
通讯作者：
Taylor, JA
中科院分区：
文献类型：
--
作者：
Slebos, RJC;Little, RE;Taylor, JA
关于低剂量辐射对人类生殖系可能的遗传毒性影响的知识是有限的，主要依赖于高剂量暴露的推断。为了测试电离辐射是否会导致父亲的基因突变传递给后代，我们招募了88名暴露在电离辐射中的切尔诺贝利清理工人的家庭。我们分析了从淋巴细胞中分离的DNA的突变，方法是用小卫星探针33.6和33.15进行多位点DNA印迹，并在一组六个四核苷酸重复序列中进行聚合酶链式反应。之前怀孕的孩子和父亲暴露后怀孕的孩子在突变频率上没有统计上的显著差异。我们看到，在辐射暴露后，生殖系微卫星突变的增加没有统计学意义。我们发现突变率不依赖于增加暴露。一个新的发现是，四核苷酸标记D7S1482表现出种系高度变异性。总而言之，我们的结果并不支持胚系小卫星突变水平的增加，但表明四核苷酸重复的胚系突变水平略有增加。然而，样本量小，统计能力有限。(C)2003爱思唯尔B.V.保留所有权利。
Knowledge about possible genotoxic effects of low-dose radiation on the human germline is limited and relies primarily on extrapolations from high-dose exposures. To test whether ionizing radiation can cause paternal genetic mutations that are transmitted to offspring, we enrolled families of 88 Chernobyl cleanup workers exposed to ionizing radiation. We analyzed DNA isolated from lymphocytes for mutations via DNA blotting with the multi-locus minisatellite probes 33.6 and 33.15 and via PCR in a panel of six tetranucleotide repeats. Children conceived before and children conceived after their father's exposure showed no statistically significant differences in mutation frequencies. We saw an increase in germline microsatellite mutations after radiation exposure that was not statistically significant. We found no dependence of mutation rate on increasing exposure. A novel finding was that the tetranucleotide marker D7S 1482 demonstrated germline hypermutability. In conclusion, our results do not support an increased level of germline minisatellite mutations but suggest a modest increase in germline mutations in tetranucleotide repeats. Small sample size, however, limited statistical power. (C) 2003 Elsevier B.V. All rights reserved.